{"refrec":{"BRefID":261943,"RR":"<b>Pierrot, C.; Péqueux, A.; Thuet, P.</b> (1995). Effects of ions substitutions and of inhibitors on transepithelial potential difference and sodium fluxes in perfused gills of the crab <i>Pachygrapsus marmoratus</i>. <i>Arch. Physiol. Biochem. 103(4)</i>: 466-475. <a href=\"https://dx.doi.org/10.3109/13813459509047141\" target=\"_blank\">https://dx.doi.org/10.3109/13813459509047141</a>","BEntID":253961,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Arch. Physiol. Biochem. 103(4)</i>: 466-475. <a href=\"https://dx.doi.org/10.3109/13813459509047141\" target=\"_blank\">https://dx.doi.org/10.3109/13813459509047141</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Pierrot, C.; Péqueux, A.; Thuet, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Pierrot, C.; Péqueux, A.; Thuet, P.","Englishabstract":"With the same saline on both sides of the epithelium, a spontaneous inside negative transepithelial potential difference (<i>PD</i>) was measured in perfused posterior gills of the euryhaline crab Pachygrapsus marmoratus acclimated to dilute sea water. The origin of the <i>PD</i> and the transport properties of the epithelium were investigated by ionic substitutions and by application of inhibitors. Diffusion ofNa<sup>+</sup> and CI' ions at the apical side contributes to the establishment of the <i>PD</i>. Sodium cyanide (10<sup>-2</sup> <i>M</i>) added to the perfusion and incubation media almost completely inhibited the transepithelial <i>PD</i> and considerably decreased the Na<sup>+</sup> influx. Internally perfused ouabain (5.10<sup>-3</sup> <i>M</i>) halved the <i>PD</i> and the Na<sup>+</sup> influx but had no effect on the Na<sup>+</sup> efflux. Externally applied amiloride (10<sup>-3</sup> M) also reduced the Na<sup>+</sup> influx by 27%. All inhibitions were concentration-dependent. From these results, it has been concluded that the transepithelial <i>PD</i> and Na<sup>+</sup> influx are, at least partly, generated by active, metabolic energy-requiring processes. The effect of ouabain supports the existence of a Na<sup>+</sup>/K<sup>+</sup> exchange mechanism linked to the presence of Na<sup>+</sup>-K<sup>+</sup> ATPase in the basolateral membrane of posterior gills. The effect of amiloride is discussed.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Effects of ions substitutions and of inhibitors on transepithelial potential difference and sodium fluxes in perfused gills of the crab <i>Pachygrapsus marmoratus</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-05-11 01:31:58.684183","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"CRAB; GILL; SODIUM FLUXES; TRANSEPITHELIAL POTENTIAL DIFFERENCE","OtherDescriptors":null,"Notes":null,"AnaPub":1995,"MonPub":null,"DateUpdate":"2022-07-12","DateCreate":"2016-10-03","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1995RW23800013","VABBcode":null,"OpenAcc":0,"DOI":"10.3109/13813459509047141"},"refs":null,"anarec":{"AnaID":261943,"PubliDate":1995,"Pagination":"466-475","XtraPublOfAnaID":null,"ISBN":null,"Volume":"103","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45852,"SerRR":"Archives of Physiology and Biochemistry. 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