{"refrec":{"BRefID":261949,"RR":"<b>Van de Casteele, M.; Legrain, C.; Wilquet, V.; Glansdorff, N.</b> (1995). The dihydrofolate reductase-encoding gene <i>dyrA</i> of the hyperthermophilic bacterium <i>Thermotoga maritima</i>. <i>Gene 158(1)</i>: 101-105. <a href=\"https://dx.doi.org/10.1016/0378-1119(95)00090-S\" target=\"_blank\">https://dx.doi.org/10.1016/0378-1119(95)00090-S</a>","BEntID":253967,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Gene 158(1)</i>: 101-105. <a href=\"https://dx.doi.org/10.1016/0378-1119(95)00090-S\" target=\"_blank\">https://dx.doi.org/10.1016/0378-1119(95)00090-S</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Van de Casteele, M.; Legrain, C.; Wilquet, V.; Glansdorff, N.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Van de Casteele, M. <i>et al.</i>","Englishabstract":"The structural gene (<i>dyrA</i>) encoding dihydrofolate reductase (DHFR) of <i>Thermotoga maritima</i> has been cloned, sequenced and expressed in <i>Escherichia coli</i>. The <i>dyrA</i> gene, located immediately upstream from the gene encoding aspartate carbamoyltransferase (<i>pyrB</i>), encodes a highly thermostable enzyme with a distinct thermophilic activity profile. Important structural features are conserved among all bacterial DHFR, yet the DHFR of <i>T. maritima</i> appears unique in a number of insertions and deletions, some of which are reminiscent of eukaryotic DHFR.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"The dihydrofolate reductase-encoding gene <i>dyrA</i> of the hyperthermophilic bacterium <i>Thermotoga maritima</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"RECOMBINANT DNA; NUCLEOTIDE SEQUENCE; EXTREME THERMOPHILE; GENEEXPRESSION","OtherDescriptors":null,"Notes":null,"AnaPub":1995,"MonPub":null,"DateUpdate":"2022-05-10","DateCreate":"2016-10-03","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1995RE87900017","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/0378-1119(95)00090-S"},"refs":null,"anarec":{"AnaID":261949,"PubliDate":1995,"Pagination":"101-105","XtraPublOfAnaID":null,"ISBN":null,"Volume":"158","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42728,"SerRR":"Gene. 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