{"refrec":{"BRefID":261952,"RR":"<b>D'Antonio, M.; Vallarino, M.; Lovejoy, D.A.; Vandesande, F.; King, J.A.; Pierantoni, R.; Peter, R.E.</b> (1995). Nature and distribution of Gonadotropin-Releasing Hormone (GnRH) in the brain, and GnRH and GnRH binding activity in serum of the spotted dogfish <i>Scyliorhinus canicula</i>. <i>Gen. Comp. Endocrinol. 98(1)</i>: 35-49. <a href=\"https://dx.doi.org/10.1006/gcen.1995.1042\" target=\"_blank\">https://dx.doi.org/10.1006/gcen.1995.1042</a>","BEntID":253970,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Gen. Comp. Endocrinol. 98(1)</i>: 35-49. <a href=\"https://dx.doi.org/10.1006/gcen.1995.1042\" target=\"_blank\">https://dx.doi.org/10.1006/gcen.1995.1042</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"D'Antonio, M.; Vallarino, M.; Lovejoy, D.A.; Vandesande, F.; King, J.A.; Pierantoni, R.; Peter, R.E.","OrigTitleTranslFlag":0,"Authorstringtrunc":"D'Antonio, M. <i>et al.</i>","Englishabstract":"The distribution of different molecular forms of gonadotropin-releasing hormone (GnRH) in the brain and serum of the spotted dogfish, <i>Scyliorhinus canicula</i>, was investigated by an indirect immunofluorescence method, using antisera against salmon (s-), chicken-II (cII-) and mammalian (m-) GnRHs, and by reverse-phase high-performance liquid chromatography (RP-HPLC) coupled with radioimmunoassays. Five GnRH molecular forms were demonstrated on the basis of the retention time in the RP-HPLC system. The characterestics of four of these GnRH peptides are consistent with those of m-, cII-, dogfish (df-), and sGnRH. The fifth form appears to be novel. Immunoreactive sGnRH structures were confined to the diencephalon; whereas cIIGnRH and mGnRH were found in the telecephalon and diencephalon, cIIGnRH-and dfGnRH-like molecules were detected in the serum. Moreover, specific, low-affinity GnRH binding protein (GnRH-BP) was found in the serum of the spotted dogfish. The binding of [<sup>125</sup>I]sGnRHA to the serum GnRH-BP was dependent on incubation time, equilibrium being reached within 1 hr at 4° binding was rapid and incompletely reversible. Scatchard analysis yielded a linear plot with a K<sub>d</sub> of 7.9 × 10<sup>-7</sup><i>M</i>. The presence of a Gnrh-BP in spotted dogfish serum suggests a probable action of GnRH via the general circulation.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Nature and distribution of Gonadotropin-Releasing Hormone (GnRH) in the brain, and GnRH and GnRH binding activity in serum of the spotted dogfish <i>Scyliorhinus canicula</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-15 01:32:56.347699","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":1995,"MonPub":null,"DateUpdate":"2022-04-15","DateCreate":"2016-10-03","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1995QQ09800004","VABBcode":null,"OpenAcc":0,"DOI":"10.1006/gcen.1995.1042"},"refs":null,"anarec":{"AnaID":261952,"PubliDate":1995,"Pagination":"35-49","XtraPublOfAnaID":null,"ISBN":null,"Volume":"98","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42729,"SerRR":"General and Comparative Endocrinology. 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