{"refrec":{"BRefID":261990,"RR":"<b>Lambert, F.; Slegers, G.</b> (1994). Preparation of bromine-77 labeled proteins using bromoperoxidase. <i>Appl. Radiat. Isot. 45(1)</i>: 11-15. <a href=\"https://dx.doi.org/10.1016/0969-8043(94)90141-4\" target=\"_blank\">https://dx.doi.org/10.1016/0969-8043(94)90141-4</a>","BEntID":254008,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Appl. Radiat. Isot. 45(1)</i>: 11-15. <a href=\"https://dx.doi.org/10.1016/0969-8043(94)90141-4\" target=\"_blank\">https://dx.doi.org/10.1016/0969-8043(94)90141-4</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Lambert, F.; Slegers, G.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Lambert, F.; Slegers, G.","Englishabstract":"The halogenating enzyme bromoperoxidase (BPO, EC 1.11.1.7), isolated from the brown algae <i>Ascophyllum nodosum</i>, was used to catalyze the radiobromination of an anti-hPLAP monoclonal antibody. Radiobromination of bovine serum albumin was also performed and used as control method. The proteins are labeled with bromine-77, produced by the <sup>75</sup>As (α, 2n) <sup>77</sup>Br reaction.The essential reaction parameters, such as concentration of protein and hydrogen peroxide, the amount of bromoperoxidase, the pH of the reaction mixture, the reaction time and temperature were optimized.The immunoreactivity of the radiolabeled monoclonal antibody was checked with a “Sandwich” ELISA.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Preparation of bromine-77 labeled proteins using bromoperoxidase","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-15 01:32:56.363325","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":1994,"MonPub":null,"DateUpdate":"2022-05-10","DateCreate":"2016-10-03","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1994MK12800003","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/0969-8043(94)90141-4"},"refs":null,"anarec":{"AnaID":261990,"PubliDate":1994,"Pagination":"11-15","XtraPublOfAnaID":null,"ISBN":null,"Volume":"45","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":258641,"SerRR":"Applied Radiation and Isotopes. Elsevier: Amsterdam.  ISSN 0969-8043; e-ISSN 1872-9800","StandardTitleSer":"Applied Radiation and Isotopes","ISSN":"0969-8043","AbbrevSer":"Appl. Radiat. Isot.","StandardTitleMon":null,"StartPage":11,"Pages":5,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Lambert","Firstname":null,"Initials":"F.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":254008,"AutID":239258,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Slegers","Firstname":null,"Initials":"G.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":254008,"AutID":239259,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":376916,"LibID":36,"BRefID":261990,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"376916","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier","City":"Amsterdam"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":254008,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":254008,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/0969-8043(94)90141-4","externalID":"10.1016/0969-8043(94)90141-4","URLTypeCode":"DOI","URLID":50626,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2016-10-03 20:16:18.820000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Chisala, Chilekwa, C.","updSesDate":{"date":"2022-05-10 08:49:24.090000","timezone_type":3,"timezone":"Europe/Brussels"}}}