{"refrec":{"BRefID":262081,"RR":"<b>Pesando, D.; Graillet, C.; Braekman, J.-C.; Dubreuil, A.; Girard, J.-P.; Puiseux-Dao, S.</b> (1991). The use of sea urchin eggs as a model to investigate the effects of crassolide, a diterpene isolated from a soft coral. <i>Toxicology in vitro 5(5-6)</i>: 395-401. <a href=\"https://dx.doi.org/10.1016/0887-2333(91)90060-Q\" target=\"_blank\">https://dx.doi.org/10.1016/0887-2333(91)90060-Q</a>","BEntID":254100,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Toxicology in vitro 5(5-6)</i>: 395-401. <a href=\"https://dx.doi.org/10.1016/0887-2333(91)90060-Q\" target=\"_blank\">https://dx.doi.org/10.1016/0887-2333(91)90060-Q</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Pesando, D.; Graillet, C.; Braekman, J.-C.; Dubreuil, A.; Girard, J.-P.; Puiseux-Dao, S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Pesando, D. <i>et al.</i>","Englishabstract":"Crassolide, a monocyclic diterpene isolated and purified from the soft coral <i>Lobophytum crassum</i>, inhibited the cell cleavage of sea urchin eggs without affecting fertilization. The effect was observed with concentrations above 2 × 10<sup>−5</sup>m in egg suspensions. Addition of crassolide between 5 and 40 min post-fertilization totally blocked the first cleavage, which in the control occurs 1 hr after fertilization. When added between 50 and 60 min post-fertilization, crassolide produced polynucleated cells in embryos. Crassolide did not affect the egg permeability to Na<sup>+</sup> and Ca<sup>2+</sup>, but caused an increase of 0.2 units in the intracellular pH of fertilized eggs coupled with a proton efflux. Crassolide, which does not affect Ca<sup>2+</sup> influx or permeability at the level of storage in reticular vesicles, could be used as a negative control when analysing calcium changes in short-term toxicological studies. The relationship between the pH increase and the cell cleavage needs further investigation.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"The use of sea urchin eggs as a model to investigate the effects of crassolide, a diterpene isolated from a soft coral","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":1991,"MonPub":null,"DateUpdate":"2022-05-10","DateCreate":"2016-10-03","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1991GU65500007","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/0887-2333(91)90060-Q"},"refs":null,"anarec":{"AnaID":262081,"PubliDate":1991,"Pagination":"395-401","XtraPublOfAnaID":null,"ISBN":null,"Volume":"5","Issue":"5-6","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":218902,"SerRR":"Toxicology in vitro. 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