{"refrec":{"BRefID":262117,"RR":"<b>Belmans, D.; Van Laere, A.</b> (1988). Glycerol-3-phosphatase and the control of glycerol metabolism in <i>Dunaliella tertiolecta</i> cells. <i>Arch. Microbiol. 150(2)</i>: 109-112. <a href=\"https://dx.doi.org/10.1007/BF00425148\" target=\"_blank\">https://dx.doi.org/10.1007/BF00425148</a>","BEntID":254136,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Arch. Microbiol. 150(2)</i>: 109-112. <a href=\"https://dx.doi.org/10.1007/BF00425148\" target=\"_blank\">https://dx.doi.org/10.1007/BF00425148</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Belmans, D.; Van Laere, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Belmans, D.; Van Laere, A.","Englishabstract":"Glycerol-3-phosphatase (EC 3.1.3.2.1) was studied by following the release of radioactive glycerol from L-(U-<sup>14</sup>C)glycerol-3-phosphate in <i>Dunaliella tertiolecta</i> enzyme extracts. The reaction showed a neutral pH optimum and had an absolute requirement for Mg<sup>2+</sup>. The substrate saturation curve was hyperbolic with an apparent <i>K</i><sub>m</sub> value for glycerol-3-phosphate of 0.7 mM in the absence of phosphate. Inorganic orthophosphate was a competitive inhibitor of the enzyme with an estimated <i>K</i><sub>j</sub> of 0.1 mM. The glycerol-3-phosphatase reaction was blocked nearly completely by millimolar Ca<sup>2+</sup> concentrations. Ca<sup>2+</sup> inhibition did not depend on the presence of calmodulin in the reaction medium. The characteristics of glycerol-3-phosphatase are discussed in relation to the regulation of the cyclic glycerol metabolism in <i>Dunaliella</i> cells during periods of osmotic stress.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Glycerol-3-phosphatase and the control of glycerol metabolism in <i>Dunaliella tertiolecta</i> cells","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-15 01:32:56.488340","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":1988,"MonPub":null,"DateUpdate":"2022-04-15","DateCreate":"2016-10-03","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1988N754800002","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/BF00425148"},"refs":null,"anarec":{"AnaID":262117,"PubliDate":1988,"Pagination":"109-112","XtraPublOfAnaID":null,"ISBN":null,"Volume":"150","Issue":"2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":133426,"SerRR":"Archives of Microbiology. 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