{"refrec":{"BRefID":262184,"RR":"<b>Roggen, E.; Dams, E.; Slegers, H.</b> (1985). The primer specificity of cytoplasmic poly(A) polymerase from cryptobiotic gastrulae of <i>Artemia salina</i>. <i>Biochim. Biophys. Acta 825(1)</i>: 21-29. <a href=\"https://dx.doi.org/10.1016/0167-4781(85)90075-2\" target=\"_blank\">https://dx.doi.org/10.1016/0167-4781(85)90075-2</a>","BEntID":254203,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Biochim. Biophys. Acta 825(1)</i>: 21-29. <a href=\"https://dx.doi.org/10.1016/0167-4781(85)90075-2\" target=\"_blank\">https://dx.doi.org/10.1016/0167-4781(85)90075-2</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Roggen, E.; Dams, E.; Slegers, H.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Roggen, E.; Dams, E.; Slegers, H.","Englishabstract":"Poly(A) polymerase has been purified to near homogeneity from the cytoplasm of <i>Artemia salina</i> as described previously (Roggen, E and Slegers, H. (1985) Eur. J. Biochem. 147, 225–232). Affinity chromatography on poly(A)-Sepharose 4B separates the enzyme preparation into two fractions. In standard assay conditions poly(A) polymerase fraction I (poly(A)-Sepharose 4B unbound) and fraction II (poly(A)-Sepharose 4B bound) have specific activities of 2.4 and 8.0 μmol AMP/h per mg enzyme, respectively. Poly(A) polymerase fraction II shows a high primer specificity towards the 17 S poly(A)-containing mRNP. Depending on the reaction conditions used, poly(A) sequences of 140 ± 15 AMP residues/μg enzyme are synthesized on the latter primer. In contrast, poly(A) polymerase fraction I only elongates oligo(A) primers efficiently. An endogenous RNA is detected in poly(A) polymerase II preparations. This RNA has a length of 83 ± 2 nucleotides and is a component of a 60 kDa particle. After removal of the latter the specificity of poly(A) polymerase fraction II for the 17 S poly(A)-containing mRNP is abolished and the characteristics of the enzyme resemble those of poly(A) polymerase I.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"The primer specificity of cytoplasmic poly(A) polymerase from cryptobiotic gastrulae of <i>Artemia salina</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-14 01:31:37.361919","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"     Poly(A) polymerase; Primer specificity; (Artemia) ","OtherDescriptors":null,"Notes":null,"AnaPub":1985,"MonPub":null,"DateUpdate":"2022-05-10","DateCreate":"2016-10-03","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1985AKR2600003","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/0167-4781(85)90075-2"},"refs":null,"anarec":{"AnaID":262184,"PubliDate":1985,"Pagination":"21-29","XtraPublOfAnaID":null,"ISBN":null,"Volume":"825","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42256,"SerRR":"Biochimica et Biophysica Acta. 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