{"refrec":{"BRefID":262205,"RR":"<b>Thoen, C.; Van Hove, L.; Piot, E.; Slegers, H.</b> (1984). Purification and characterization of the messenger ribonucleoprotein-associated casein kinase II of <i>Artemia salina</i> cryptobiotic gastrulae. <i>Biochim. Biophys. Acta 783(2)</i>: 105-113. <a href=\"https://dx.doi.org/10.1016/0167-4781(84)90001-0\" target=\"_blank\">https://dx.doi.org/10.1016/0167-4781(84)90001-0</a>","BEntID":254224,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Biochim. Biophys. Acta 783(2)</i>: 105-113. <a href=\"https://dx.doi.org/10.1016/0167-4781(84)90001-0\" target=\"_blank\">https://dx.doi.org/10.1016/0167-4781(84)90001-0</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Thoen, C.; Van Hove, L.; Piot, E.; Slegers, H.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Thoen, C. <i>et al.</i>","Englishabstract":"The mRNP-associated protein kinase is purified to near homogeneity by ion-exchange chromatography on phosphocellulose and affinity chromatography on casein-Sepharose 4B and ATP-agarose. The cyclic nucleotide-independent enzyme phosphorylates casein using either ATP or GTP. The enzyme exists in two forms composed of subunits with <i>M</i><sub>r</sub> 36 500 (α) and 28 000 (β) and of subunits with <i>M</i><sub>r</sub> 36 500 (α), 33 000 (α′) and 28 000 (β). The undegraded enzyme has an <i>M</i><sub>r</sub> of 136 000 ± 7000. The enzyme is inhibited by heparin and hemin and stimulated by spermine. The mRNP-associated protein kinase may be classified as a casein kinase II. Main mRNP protein phosphate acceptors have <i>M</i><sub>r</sub> values of 112 000, 72 000, 65 000, 53 000, 38 000, 28 000, 23 500 and 21 000. Phosphorylation of the <i>M</i><sub>r</sub> 38 000 poly(A)-binding protein resulted in the generation of different acidic ionic species. From the observed inhibition of the translational activity after phosphorylation by the mRNP-associated protein kinase a function in the repression of mRNP is proposed.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Purification and characterization of the messenger ribonucleoprotein-associated casein kinase II of <i>Artemia salina</i> cryptobiotic gastrulae","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-05-29 01:31:16.014845","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"     Messenger ribonucleoprotein; Protein kinase; Subunit composition; (A. salina) ","OtherDescriptors":null,"Notes":null,"AnaPub":1984,"MonPub":null,"DateUpdate":"2022-05-10","DateCreate":"2016-10-03","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1984TY58200001","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/0167-4781(84)90001-0"},"refs":null,"anarec":{"AnaID":262205,"PubliDate":1984,"Pagination":"105-113","XtraPublOfAnaID":null,"ISBN":null,"Volume":"783","Issue":"2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42256,"SerRR":"Biochimica et Biophysica Acta. 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