{"refrec":{"BRefID":27451,"RR":"<b>Yang, S.; Wu, R.S.S.; Kong, R.Y.C.</b> (2002). Physiological and cytological responses of the marine diatom <i>Skeletonema costatum</i> to 2,4-dichlorophenol. <i>Aquat. Toxicol. 60(1-2)</i>: 33-41. <a href=\"https://dx.doi.org/10.1016/S0166-445X(01)00258-2\" target=\"_blank\">https://dx.doi.org/10.1016/S0166-445X(01)00258-2</a>","BEntID":27451,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquat. Toxicol. 60(1-2)</i>: 33-41. <a href=\"https://dx.doi.org/10.1016/S0166-445X(01)00258-2\" target=\"_blank\">https://dx.doi.org/10.1016/S0166-445X(01)00258-2</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Yang, S.; Wu, R.S.S.; Kong, R.Y.C.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Yang, S.; Wu, R.S.S.; Kong, R.Y.C.","Englishabstract":"Physiological and cytological responses of the marine diatom, <i>Skeletonema costatum</i>, to 2,4-dichlorophenol (2,4-DCP) were investigated after exposing algal cultures to 1-6 mg<sup>-1</sup> of 2,4-DCP for 96 h. Growth rate of the diatom was significantly reduced by 2,4-DCP at and above 3.0 mg<sup>-1</sup> while other biological parameters such as photosynthetic and respiration rates, carotenoid and protein content, ATP level and adenylate energy charge were unaffected. Exposure to 6.0 mg<sup>-1</sup> 2,4-DCP for 96 h resulted in the total lipid content being increased to 304%, while the RNA/DNA ratio was reduced to 31% of the control values. A parallel study by transmission electron microscopy further confirmed the increase in cellular lipid content, as evidenced by the accumulation of lipid droplets within diatom cells. A slight increase in carbohydrate (+37.8%) and decrease in chlorophyll a (20.4%) and total chlorophyll c (14.4%) were also found at 6.0 mg<sup>-1</sup> 2,4-DCP. Although 2,4-DCP is known to uncouple oxidative phosphorylation, our results show that energy production was not significantly inhibited at sublethal concentrations of 2,4-DCP. The observed growth inhibition in <i>S. costatum</i> caused by 2,4-DCP was associated with an increase in energy storage and inhibition of protein synthesis, as indicated by a reduction in RNA/DNA ratio.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Physiological and cytological responses of the marine diatom <i>Skeletonema costatum</i> to 2,4-dichlorophenol","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-05-12 01:31:35.719450","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2002,"MonPub":null,"DateUpdate":"2002-09-24","DateCreate":"2002-09-24","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000178066600003","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/S0166-445X(01)00258-2"},"refs":null,"anarec":{"AnaID":27451,"PubliDate":2002,"Pagination":"33-41","XtraPublOfAnaID":null,"ISBN":null,"Volume":"60","Issue":"1-2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. 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