{"refrec":{"BRefID":280371,"RR":"<b>De Herdt, E.; Piot, E.; Kondo, M.; Slegers, H.</b> (1982). The 38000-Mr poly(A)-binding protein of non-polysomal messenger ribonucleoproteins of cryptobiotic gastrulae of <i>Artemia salina</i>. <i>Eur. J. Biochem. 122(3)</i>: 453-460. <a href=\"http://dx.doi.org/10.1111/j.1432-1033.1982.tb06459.x\" target=\"_blank\">http://dx.doi.org/10.1111/j.1432-1033.1982.tb06459.x</a>","BEntID":272390,"PublicFlag":1,"CheckedFlag":1,"wosflag":null,"vabbflag":null,"RefStringPartII":". <i>Eur. J. Biochem. 122(3)</i>: 453-460. <a href=\"http://dx.doi.org/10.1111/j.1432-1033.1982.tb06459.x\" target=\"_blank\">http://dx.doi.org/10.1111/j.1432-1033.1982.tb06459.x</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"De Herdt, E.; Piot, E.; Kondo, M.; Slegers, H.","OrigTitleTranslFlag":0,"Authorstringtrunc":"De Herdt, E. <i>et al.</i>","Englishabstract":"The 38000-M<sub>r</sub> poly(A)-binding protein has been purified to near homogeneity from non-polysomal messenger ribonucleoprotein of <i>Artemia salina</i> [Slegers, H., De Herdt, E., and Kondo, M. (1981) <i>Eur. J. Biochem.</i> 117, 111-120]. The protein consists of approximately 357 amino acids and is characterized by a high glycine content of 22.5% and the presence of dimethylarginine. From polynucleotide-protein binding experiments a stoichiometry of 9–11 adenylate and 10–12 uridylate residues per protein molecule is calculated. The polypeptide is devoid of poly(A) polymerase and RNase activities. The poly(A)-binding protein and the helix-destabilizing protein HD40 [Marvil, D. K., Nowak, L., and Szer, W. (1980) <i>J. Biol. Chem.</i> 255, 6466–6472] have the same mobility in polyacrylamide/dodecylsulphate gel electrophoresis and exhibit a comparable amino acid composition and protein-polynucleotide stoichiometry. Based on the length of poly(A) sequences of mRNA and from protein-poly(A) binding experiments, a repetitive binding of the 38000-M<sub>r</sub> protein on the poly(A) sequence is demonstrated. The 38000-M<sub>r</sub> protein of cytoplasmic and membrane-bound non-polysomal messenger ribonucleoproteins is also compared.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"The 38000-Mr poly(A)-binding protein of non-polysomal messenger ribonucleoproteins of cryptobiotic gastrulae of <i>Artemia salina</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-15 01:32:56.925877","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":1982,"MonPub":null,"DateUpdate":"2016-11-21","DateCreate":"2016-10-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1982NF04400003","VABBcode":null,"OpenAcc":1,"DOI":"10.1111/j.1432-1033.1982.tb06459.x"},"refs":null,"anarec":{"AnaID":280371,"PubliDate":1982,"Pagination":"453-460","XtraPublOfAnaID":null,"ISBN":null,"Volume":"122","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42671,"SerRR":"European Journal of Biochemistry. Springer: Berlin.  ISSN 0014-2956; e-ISSN 1432-1033","StandardTitleSer":"European Journal of Biochemistry","ISSN":"0014-2956","AbbrevSer":"Eur. J. 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