{"refrec":{"BRefID":280394,"RR":"<b>Slegers, H.; De Herdt, E.; Kondo, M.</b> (1981). Non-polysomal poly(A)-containing messenger ribonucleoproteins of cryptobiotic gastrulae of <i>Artemia salina</i>. <i>Eur. J. Biochem. 117(1)</i>: 111-120. <a href=\"http://dx.doi.org/10.1111/j.1432-1033.1981.tb06309.x\" target=\"_blank\">http://dx.doi.org/10.1111/j.1432-1033.1981.tb06309.x</a>","BEntID":272413,"PublicFlag":1,"CheckedFlag":1,"wosflag":null,"vabbflag":null,"RefStringPartII":". <i>Eur. J. Biochem. 117(1)</i>: 111-120. <a href=\"http://dx.doi.org/10.1111/j.1432-1033.1981.tb06309.x\" target=\"_blank\">http://dx.doi.org/10.1111/j.1432-1033.1981.tb06309.x</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Slegers, H.; De Herdt, E.; Kondo, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Slegers, H. <i>et al.</i>","Englishabstract":"Non-polysomal poly(A)-containing messenger ribonucleoprotein (mRNP) of <i>Artemia salina</i> has been isolated by thermal chromatography on oligo(dT)-cellulose in moderate (250 mM) and low (50 mM NaCl and 5 mM MgCl<sub>2</sub>) ionic strength. The purified particles sedimented between 5 S and 30 S and banded at a density of 1.38–1.40 g/cm<sup>3</sup> and 1.26–1.27 g/cm<sup>3</sup> in CsCl and sucrose isopycnic centrifugation, respectively. The translatability of the mRNP in a cell-free system depended on the conditions of isolation. The protein composition of the free mRNP is independent of the conditions used in oligo(dT)-cellulose chromatography. The proteins have <i>M</i><sub>r</sub> of 87000, 76000, 65000, 50000, 45000, 38000 and 23500. A specific set of proteins is associated with different ribonucleoproteins, although some proteins are present on multiple particles. The main 17 ± 2-S particle is composed of proteins with <i>M</i><sub>r</sub> of 87000, 76000, 45000 and 38000. Approximately the same proteins were present on free mRNP and mRNP isolated from non-polysomal mRNP-ribosome complexes. Poly(A)-binding proteins have <i>M</i><sub>r</sub> of 38000 and 23500. The 38000-<i>M</i><sub>r</sub> protein comprised at least 60% of the total mRNP protein. Poly(A)-binding proteins with <i>M</i><sub>r</sub> of 38000 and 76000 are also present in a free state in the cytoplasm. A relation between the main poly(A)-binding mRNP protein and the helix-destabilizing protein HD40 [Marvil, D. K., Nowak, L., and Szer, W. (1980) <i>J. Biol. Chem. 255</i>, 6466–6472] is discussed.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Non-polysomal poly(A)-containing messenger ribonucleoproteins of cryptobiotic gastrulae of <i>Artemia salina</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-15 01:32:56.957137","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":1981,"MonPub":null,"DateUpdate":"2016-11-28","DateCreate":"2016-10-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1981LW27700016","VABBcode":null,"OpenAcc":1,"DOI":"10.1111/j.1432-1033.1981.tb06309.x"},"refs":null,"anarec":{"AnaID":280394,"PubliDate":1981,"Pagination":"111-120","XtraPublOfAnaID":null,"ISBN":null,"Volume":"117","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42671,"SerRR":"European Journal of Biochemistry. 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