{"refrec":{"BRefID":280464,"RR":"<b>Slegers, H.; De Herdt, E.; Kondo, M.</b> (1979). Characterization of protein components of poly(A)-containing messenger ribonucleoproteins from cryptobiotic gastrulae of <i>Artemia salina</i>. <i>Molecular biology reports 5(1-2)</i>: 65-69. <a href=\"https://dx.doi.org/10.1007/BF00777490\" target=\"_blank\">https://dx.doi.org/10.1007/BF00777490</a>","BEntID":272483,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Molecular biology reports 5(1-2)</i>: 65-69. <a href=\"https://dx.doi.org/10.1007/BF00777490\" target=\"_blank\">https://dx.doi.org/10.1007/BF00777490</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Slegers, H.; De Herdt, E.; Kondo, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Slegers, H.; De Herdt, E.; Kondo, M.","Englishabstract":"Conditions for the isolation of intact poly(A)<sup>+</sup>mRNP from cryptobiotic gastrulae of <i>A. salina</i> are described. In the presence of Mg<sup>2+</sup> ions nucleolytic cleavage occurs in vitro in the vicinity of the 3′-poly(A) segment of mRNP during the isolation procedure. The resulting two parts of poly(A)<sup>+</sup>mRNP complex are separated by thermal elution from oligo(dT)-cellulose affinity column. Analysis by SDS-gel electrophoresis of protein components associated with intact poly(A)<sup>+</sup>mRNP has revealed the existence of 20–30 S RNP complex containing five major proteins with M<sub>r</sub> 68,000, 53,000, 50,000, 45,000 and 38,000, respectively, but completely lacking the poly(A)-specific M<sub>r</sub> 76,000 protein.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Characterization of protein components of poly(A)-containing messenger ribonucleoproteins from cryptobiotic gastrulae of <i>Artemia salina</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":1979,"MonPub":null,"DateUpdate":"2022-04-15","DateCreate":"2016-10-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1979HB03000008","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/BF00777490"},"refs":null,"anarec":{"AnaID":280464,"PubliDate":1979,"Pagination":"65-69","XtraPublOfAnaID":null,"ISBN":null,"Volume":"5","Issue":"1-2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":177286,"SerRR":"Molecular biology reports. 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