{"refrec":{"BRefID":280531,"RR":"<b>Slegers, H.; Kondo, M.</b> (1977). Messenger ribonucleoprotein complexes of cryptobiotic embryos of <i>Artemia salina</i>. <i>Nucleic Acids Res. 4(3)</i>: 625-639. <a href=\"https://dx.doi.org/10.1093/nar/4.3.625\" target=\"_blank\">https://dx.doi.org/10.1093/nar/4.3.625</a>","BEntID":272550,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Nucleic Acids Res. 4(3)</i>: 625-639. <a href=\"https://dx.doi.org/10.1093/nar/4.3.625\" target=\"_blank\">https://dx.doi.org/10.1093/nar/4.3.625</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Slegers, H.; Kondo, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Slegers, H.; Kondo, M.","Englishabstract":"Poly(A)-containing ribonucleoprotein [poly(A)+•RNP) particles in the post-mitochondrial supernatant of cryptobiotic embryos of Artemia salina were characterized by hybridization to [<sup>3</sup>H]poly(U). By sucrose isopycnic centrifugation, approximately 2/3 of poly(A)+•RNPs was found to band at 1.27–1.30 (g/cm<sup>3</sup>) and the rest 1/3 at 1.20–1.23 (g/cm<sup>3</sup>) and below 1.20 (g/cm<sup>3</sup>). The 1.27–1.30 RNP8 could be separated into two density classes, 1.27–1.26 and 1.30 (g/cm<sup>3</sup>) respectively. The latter RNP class was apparently complexed with ribosomal components because they were completely converted to the former RNP class [free RNPs) by 25 mM EDTA treatment. Further, the 1.30 (g/cm<sup>3</sup>) RNPs were res-solved into several RNP species having sedimentation coefficients above 50 S, which were transformed mostly to 20–30 S RNPs in the presence of 25 mM EDTA. 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