{"refrec":{"BRefID":280623,"RR":"<b>Phuoc, L.H.; Hu, B.; Wille, M.; Hien, N.T.; Phuong, V.H.; Tinh, N.T.N.; Loc, N.H.; Sorgeloos, P.; Bossier, P.</b> (2016). Priming the immune system of Penaeid shrimp by bacterial HSP70 (DnaK). <i>J. Fish Dis. 39(5)</i>: 555-564. <a href=\"http://dx.doi.org/10.1111/jfd.12388\" target=\"_blank\">dx.doi.org/10.1111/jfd.12388</a>","BEntID":272642,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>J. Fish Dis. 39(5)</i>: 555-564. <a href=\"https://dx.doi.org/10.1111/jfd.12388\" target=\"_blank\">https://dx.doi.org/10.1111/jfd.12388</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Phuoc, L.H.; Hu, B.; Wille, M.; Hien, N.T.; Phuong, V.H.; Tinh, N.T.N.; Loc, N.H.; Sorgeloos, P.; Bossier, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Phuoc, L.H. <i>et al.</i>","Englishabstract":"This study was conducted to test the effect of DnaK on priming immune responses in Penaeid shrimp. Juvenile-specific pathogen-free (SPF) <i>P. vannamei</i> shrimp were injected with 0.05 μg recombinant DnaK. One hour post-DnaK priming, a non-lethal dose of <i>Vibrio campbellii</i> (10<sup>5 </sup>CFU shrimp<sup>−1</sup>) was injected. Other treatments include only DnaK or <i>V. campbellii</i> injection or control with blank inocula. The haemolymph of three shrimp from each treatment was collected at 1.5, 6, 9 and 12 h post-DnaK priming (hpp). It was verified that injection with DnaK and <i>V. campbellii</i> challenge affected the transcription of 3 immune genes, transglutaminase-1 (TGase-1), prophenoloxidase-2 (proPO-2) and endogenous HSP70 (lvHSP70). In <i>P. monodon</i>, shrimp were first injected with DnaK at a dose of 10 μg shrimp<sup>−1</sup> and one hour later with 10<sup>6 </sup>CFU of <i>V. harveyi</i> (BB120) shrimp<sup>−1</sup>. Shrimp injected with DnaK showed a significant increase in proPO expression compared to the control (<i>P</i> < 0.05). Yet a double injection (DnaK and <i>Vibrio</i>) seemed to cause an antagonistic response at the level of expression, which was not equalled at the level of PO activity. Those results suggest that DnaK is able to modulate immune responses in <i>P. vannamei</i> and <i>P. monodon</i>.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Priming the immune system of Penaeid shrimp by bacterial HSP70 (DnaK)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"DnaK heat-shock proteins; heat-shock protein; Penaeus monodon; Penaeusvannamei; recombinant protein; Vibrio","OtherDescriptors":null,"Notes":null,"AnaPub":2016,"MonPub":null,"DateUpdate":"2016-10-18","DateCreate":"2016-10-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000374555900005","VABBcode":null,"OpenAcc":0,"DOI":"10.1111/jfd.12388"},"refs":null,"anarec":{"AnaID":280623,"PubliDate":2016,"Pagination":"555-564","XtraPublOfAnaID":null,"ISBN":null,"Volume":"39","Issue":"5","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":44476,"SerRR":"Journal of Fish Diseases. Blackwell Science: Oxford; London; Edinburgh; Boston; Melbourne.  ISSN 0140-7775; e-ISSN 1365-2761","StandardTitleSer":"Journal of Fish Diseases","ISSN":"0140-7775","AbbrevSer":"J. 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