{"refrec":{"BRefID":280624,"RR":"<b>Sinnasamy, S.; Noordin, N.M.; MacRae, T.H.; bin Abdullah, M.I.; Bossier, P.; Wahid, M.E.B.; Noriaki, A.; Sung, Y.Y.</b> (2016). Ingestion of food pellets containing <i>Escherichia coli</i> overexpressing the heat-shock protein DnaK protects <i>Penaeus vannamei</i> (Boone) against <i>Vibrio harveyi</i> (Baumann) infection. <i>J. Fish Dis. 39(5)</i>: 577-584. <a href=\"http://dx.doi.org/10.1111/jfd.12390\" target=\"_blank\">dx.doi.org/10.1111/jfd.12390</a>","BEntID":272643,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>J. Fish Dis. 39(5)</i>: 577-584. <a href=\"https://dx.doi.org/10.1111/jfd.12390\" target=\"_blank\">https://dx.doi.org/10.1111/jfd.12390</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Sinnasamy, S.; Noordin, N.M.; MacRae, T.H.; bin Abdullah, M.I.; Bossier, P.; Wahid, M.E.B.; Noriaki, A.; Sung, Y.Y.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Sinnasamy, S. <i>et al.</i>","Englishabstract":"Feeding aquatic animals with bacterial encapsulated heat-shock proteins (Hsps) is potentially a new method to combat vibriosis, an important disease affecting aquatic animals used in aquaculture. Food pellets comprised of shrimp and containing <i>Escherichia coli</i> overexpressing either DnaK-DnaJ-GrpE, the prokaryotic equivalents of Hsp70-Hsp40-Hsp20, or only DnaK were fed to juveniles of the white leg shrimp <i>Penaeus vannamei</i>, and protection against pathogenic <i>Vibrio harveyi</i> was determined. Maintaining pellets at different temperatures for varying lengths of time reduced the number of live adhering <i>E. coli</i>, as did contact with sea water, demonstrating that storage and immersion adversely affected bacterial survival and attachment to pellets. Feeding <i>P. vannamei</i> with <i>E. coli</i> did not compromise their survival, indicating that the bacteria were not pathogenic to shrimp. Feeding <i>P. vannamei</i> with pellets containing bacteria overproducing DnaK (approximately 60 cells g<sup>−1</sup> pellets) boosted <i>P. vannamei</i> survival twofold against <i>V. harveyi</i>, suggesting that DnaK plays a role in <i>Vibrio</i> tolerance. Pellets containing DnaK were effective in providing protection to <i>P. vannamei</i> for up to 2 weeks before loss of viability and that DnaK encapsulated by these bacteria enhanced shrimp resistance against <i>Vibrio</i> infection.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Ingestion of food pellets containing <i>Escherichia coli</i> overexpressing the heat-shock protein DnaK protects <i>Penaeus vannamei</i> (Boone) against <i>Vibrio harveyi</i> (Baumann) infection","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"DnaK; heat-shock proteins; Hsp70; P. vannamei; shrimp; Vibrio","OtherDescriptors":null,"Notes":null,"AnaPub":2016,"MonPub":null,"DateUpdate":"2016-10-19","DateCreate":"2016-10-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000374555900007","VABBcode":null,"OpenAcc":0,"DOI":"10.1111/jfd.12390"},"refs":null,"anarec":{"AnaID":280624,"PubliDate":2016,"Pagination":"577-584","XtraPublOfAnaID":null,"ISBN":null,"Volume":"39","Issue":"5","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":44476,"SerRR":"Journal of Fish Diseases. 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