{"refrec":{"BRefID":280628,"RR":"<b>García-Fernández, R.; Peigneur, S.; Pons, T.; Alvarez, C.; González, L.; Chávez, M.A.; Tytgat, J.</b> (2016). The Kunitz-type protein ShPI-1 inhibits serine proteases and voltage-gated potassium channels. <i>Toxins 8(4)</i>: 110. <a href=\"http://dx.doi.org/10.3390/toxins8040110\" target=\"_blank\">dx.doi.org/10.3390/toxins8040110</a>","BEntID":272647,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Toxins 8(4)</i>: 110. <a href=\"https://dx.doi.org/10.3390/toxins8040110\" target=\"_blank\">https://dx.doi.org/10.3390/toxins8040110</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"García-Fernández, R.; Peigneur, S.; Pons, T.; Alvarez, C.; González, L.; Chávez, M.A.; Tytgat, J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"García-Fernández, R. <i>et al.</i>","Englishabstract":"The bovine pancreatic trypsin inhibitor (BPTI)-Kunitz-type protein ShPI-1 (UniProt: P31713) is the major protease inhibitor from the sea anemone <i>Stichodactyla helianthus</i>. This molecule is used in biotechnology and has biomedical potential related to its anti-parasitic effect. A pseudo wild-type variant, <i>r</i>ShPI-1A, with additional residues at the <i>N</i>- and <i>C</i>-terminal, has a similar three-dimensional structure and comparable trypsin inhibition strength. Further insights into the structure-function relationship of <i>r</i>ShPI-1A are required in order to obtain a better understanding of the mechanism of action of this sea anemone peptide. Using enzyme kinetics, we now investigated its activity against other serine proteases. Considering previous reports of bifunctional Kunitz-type proteins from anemones, we also studied the effect of <i>r</i>ShPI-1A on voltage-gated potassium (Kv) channels. <i>r</i>ShPI-1A binds Kv1.1, Kv1.2, and Kv1.6 channels with IC<sub>50</sub> values in the nM range. Hence, ShPI-1 is the first member of the sea anemone type 2 potassium channel toxins family with tight-binding potency against several proteases and different Kv1 channels. In depth sequence analysis and structural comparison of ShPI-1 with similar protease inhibitors and Kv channel toxins showed apparent non-sequence conservation for known key residues. However, we detected two subtle patterns of coordinated amino acid substitutions flanking the conserved cysteine residues at the <i>N</i>- and <i>C</i>-terminal ends.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"The Kunitz-type protein ShPI-1 inhibits serine proteases and voltage-gated potassium channels","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"protease inhibitor; Kv channel inhibitor; sea anemone; toxin;Kunitz-type protein","OtherDescriptors":null,"Notes":null,"AnaPub":2016,"MonPub":null,"DateUpdate":"2016-10-18","DateCreate":"2016-10-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000375861700025","VABBcode":null,"OpenAcc":1,"DOI":"10.3390/toxins8040110"},"refs":null,"anarec":{"AnaID":280628,"PubliDate":2016,"Pagination":"110","XtraPublOfAnaID":null,"ISBN":null,"Volume":"8","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":197331,"SerRR":"Toxins. Multidisciplinary Digital Publishing Institute (MDPI): Basel.  e-ISSN 2072-6651","StandardTitleSer":"Toxins","ISSN":null,"AbbrevSer":"Toxins","StandardTitleMon":null,"StartPage":110,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"García-Fernández","Firstname":"Rossana","Initials":"R.","Affiliation":"Univ La Habana, Fac Biol, Ctr Estudio Prot, Calle 25 455, Havana 10400, Cuba.","Discriminator":null,"CorporateFlag":0,"BEntID":272647,"AutID":240173,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Peigneur","Firstname":"Steve","Initials":"S.","Affiliation":"Laboratory of Toxicology and Pharmacology, KU Leuven","Discriminator":null,"CorporateFlag":0,"BEntID":272647,"AutID":242977,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"KU Leuven; Departement Farmaceutische en Farmacologische Wetenschappen; Laboratorium voor Toxicologie en Farmacologie","ORCID":"0000-0003-0504-5702","PersID":27019,"InsID":492},{"AutName":"Pons","Firstname":"Tirso","Initials":"T.","Affiliation":"Spanish Natl Canc Res Ctr CNIO, Struct Biol & Biocomp Programme, C Melchor Fernandez Almagro 3, E-28029 Madrid, Spain.","Discriminator":null,"CorporateFlag":0,"BEntID":272647,"AutID":240175,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Alvarez","Firstname":"Carlos","Initials":"C.","Affiliation":"Univ La Habana, Fac Biol, Ctr Estudio Prot, Calle 25 455, Havana 10400, Cuba.","Discriminator":null,"CorporateFlag":0,"BEntID":272647,"AutID":240176,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"González","Firstname":"Lidice","Initials":"L.","Affiliation":"Univ La Habana, Fac Biol, Ctr Estudio Prot, Calle 25 455, Havana 10400, Cuba.","Discriminator":null,"CorporateFlag":0,"BEntID":272647,"AutID":240177,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Chávez","Firstname":"María","Initials":"M.A.","Affiliation":"Univ La Habana, Fac Biol, Ctr Estudio Prot, Calle 25 455, Havana 10400, Cuba.","Discriminator":null,"CorporateFlag":0,"BEntID":272647,"AutID":240178,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Tytgat","Firstname":"Jan","Initials":"J.","Affiliation":"Laboratory of Toxicology and Pharmacology, KU Leuven","Discriminator":null,"CorporateFlag":0,"BEntID":272647,"AutID":240179,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"KU Leuven; Departement Farmaceutische en Farmacologische Wetenschappen; Laboratorium voor Toxicologie en Farmacologie","ORCID":null,"PersID":717,"InsID":492}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":295986,"LibID":36,"BRefID":280628,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"295986","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Multidisciplinary Digital Publishing Institute (MDPI)","City":"Basel"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Stichodactyla helianthus"}],"geotermsASFA":null,"collections":[{"Collection":"OMA - Open Marien Archief","ShortName":"OMA"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"},"221":{"SpName":"OMA - Open Marien Archief","SpColID":221,"ParSpColID":null,"TopParID":null,"ShortName":"OMA","URLLocation":null,"LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"OMA"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":272647,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":272647,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.3390/toxins8040110","externalID":"10.3390/toxins8040110","URLTypeCode":"DOI","URLID":51004,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Stichodactyla helianthus","AphiaID":291135,"TaxtID":42823}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Stichodactyla helianthus","DutchTerm":null,"AphiaID":291135,"TaxtID":42823}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2016-10-09 21:07:26.660000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2016-10-18 09:15:20.940000","timezone_type":3,"timezone":"Europe/Brussels"}}}