{"refrec":{"BRefID":280643,"RR":"<b>Ruwandeepika, H.A.D.; Karunasagar, I.; Bossier, P.; Defoirdt, T.</b> (2015). Expression and quorum sensing regulation of type III secretion system genes of <i>Vibrio harveyi</i> during infection of gnotobiotic brine shrimp. <i>PLoS One 10(12)</i>: e0143935. <a href=\"https://dx.doi.org/10.1371/journal.pone.0143935\" target=\"_blank\">https://dx.doi.org/10.1371/journal.pone.0143935</a>","BEntID":272662,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>PLoS One 10(12)</i>: e0143935. <a href=\"https://dx.doi.org/10.1371/journal.pone.0143935\" target=\"_blank\">https://dx.doi.org/10.1371/journal.pone.0143935</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Ruwandeepika, H.A.D.; Karunasagar, I.; Bossier, P.; Defoirdt, T.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Ruwandeepika, H.A.D. <i>et al.</i>","Englishabstract":"Type III secretion systems enable pathogens to inject their virulence factors directly into the cytoplasm of the host cells. The type III secretion system of <i>Vibrio harveyi</i>, a major pathogen of aquatic organisms and a model species in quorum sensing studies, is repressed by the quorum sensing master regulator LuxR. In this study, we found that during infection of gnotobiotic brine shrimp larvae, the expression levels of three type III secretion operons in <i>V</i>. <i>harveyi</i> increased within the first 12h after challenge and decreased again thereafter. The <i>in vivo</i> expression levels were highest in a mutant with a quorum sensing system that is locked in low cell density configuration (minimal LuxR levels) and lowest in a mutant with a quorum sensing system that is locked in the high cell density configuration (maximal LuxR levels), which is consistent with repression of type III secretion by LuxR. Remarkably, <i>in vivo</i> expression levels of the type III secretion system genes were much (> 1000 fold) higher than the <i>in vitro</i> expression levels, indicating that (currently unknown) host factors significantly induce the type III secretion system. Given the fact that type III secretion is energy-consuming, repression by the quorum sensing master regulators might be a mechanism to save energy under conditions where it does not provide an advantage to the cells.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Expression and quorum sensing regulation of type III secretion system genes of <i>Vibrio harveyi</i> during infection of gnotobiotic brine shrimp","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2015,"MonPub":null,"DateUpdate":"2017-06-22","DateCreate":"2016-10-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000366143800034","VABBcode":null,"OpenAcc":1,"DOI":"10.1371/journal.pone.0143935"},"refs":null,"anarec":{"AnaID":280643,"PubliDate":2015,"Pagination":"e0143935","XtraPublOfAnaID":null,"ISBN":null,"Volume":"10","Issue":"12","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":123954,"SerRR":"PLoS One. Public Library of Science: San Francisco.  ISSN 1932-6203; e-ISSN 1932-6203","StandardTitleSer":"PLoS One","ISSN":"1932-6203","AbbrevSer":"PLoS One","StandardTitleMon":null,"StartPage":null,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Ruwandeepika","Firstname":"H. A. Darshanee","Initials":"H.A.D.","Affiliation":"Sabaragamuwa Univ Sri Lanka, Fac Agr Sci, Dept Livestock Prod, Belihuloya, Sri Lanka.","Discriminator":null,"CorporateFlag":0,"BEntID":272662,"AutID":240269,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":33481,"InsID":null},{"AutName":"Karunasagar","Firstname":"Indrani","Initials":"I.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":272662,"AutID":231209,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Bossier","Firstname":"Peter","Initials":"P.","Affiliation":"Laboratory of Aquaculture & Artemia Reference Center, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":272662,"AutID":141842,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ARC","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Aquacultuur en Artemia Reference Center","ORCID":"0000-0002-6165-9111","PersID":125,"InsID":22},{"AutName":"Defoirdt","Firstname":"Tom","Initials":"T.","Affiliation":"Laboratory of Aquaculture & Artemia Reference Center, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":272662,"AutID":145686,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ARC","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Aquacultuur en Artemia Reference Center","ORCID":"0000-0002-7446-2246","PersID":13163,"InsID":22}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":303288,"LibID":36,"BRefID":280643,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"303288","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Public Library of Science","City":"San Francisco"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":[{"Collection":"OMA - Open Marien Archief","ShortName":"OMA"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"},"221":{"SpName":"OMA - Open Marien Archief","SpColID":221,"ParSpColID":null,"TopParID":null,"ShortName":"OMA","URLLocation":null,"LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"OMA"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":272662,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":272662,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1371/journal.pone.0143935","externalID":"10.1371/journal.pone.0143935","URLTypeCode":"DOI","URLID":55982,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2016-10-09 21:07:26.660000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2017-06-22 09:43:34.003000","timezone_type":3,"timezone":"Europe/Brussels"}}}