{"refrec":{"BRefID":280693,"RR":"<b>Ascenzi, P.; Pesce, A.; Nardini, M.; Bolognesi, M.; Ciaccio, C.; Coletta, M.; Dewilde, S.</b> (2013). Reductive nitrosylation of <i>Methanosarcina acetivorans</i> protoglobin: a comparative study. <i>Biochem. Biophys. Res. Commun. 430(4)</i>: 1301-1305. <a href=\"https://dx.doi.org/10.1016/j.bbrc.2012.11.122\" target=\"_blank\">https://dx.doi.org/10.1016/j.bbrc.2012.11.122</a>","BEntID":272712,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Biochem. Biophys. Res. Commun. 430(4)</i>: 1301-1305. <a href=\"https://dx.doi.org/10.1016/j.bbrc.2012.11.122\" target=\"_blank\">https://dx.doi.org/10.1016/j.bbrc.2012.11.122</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Ascenzi, P.; Pesce, A.; Nardini, M.; Bolognesi, M.; Ciaccio, C.; Coletta, M.; Dewilde, S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Ascenzi, P. <i>et al.</i>","Englishabstract":"<i>Methanosarcina acetivorans</i> is a strictly anaerobic non-motile methane-producing Archaea expressing protoglobin (Pgb) which might either facilitate O<sub>2</sub> detoxification or act as a CO sensor/supplier in methanogenesis. Unusually, <i>M. acetivorans</i> Pgb (<i>Ma</i>Pgb) binds preferentially O<sub>2</sub> rather than CO and displays anticooperativity in ligand binding. Here, kinetics and/or thermodynamics of ferric and ferrous <i>Ma</i>Pgb (<i>Ma</i>Pgb(III) and <i>Ma</i>Pgb(II), respectively) nitrosylation are reported. Data were obtained between pH 7.2 and 9.5, at 22.0 °C. Addition of NO to <i>Ma</i>Pgb(III) leads to the transient formation of <i>Ma</i>Pgb(III)–NO in equilibrium with <i>Ma</i>Pgb(II)–NO<sup>+</sup>. In turn, <i>Ma</i>Pgb(II)–NO<sup>+</sup> is converted to <i>Ma</i>Pgb(II) by OH<sup>−</sup>-based catalysis. Then, <i>Ma</i>Pgb(II) binds NO very rapidly leading to <i>Ma</i>Pgb(II)–NO. The rate-limiting step for reductive nitrosylation of <i>Ma</i>Pgb(III) is represented by the OH<sup>−</sup>-mediated reduction of <i>Ma</i>Pgb(II)–NO<sup>+</sup> to <i>Ma</i>Pgb(II). Present results highlight the potential role of <i>Ma</i>Pgb in scavenging of reactive nitrogen and oxygen species.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Reductive nitrosylation of <i>Methanosarcina acetivorans</i> protoglobin: a comparative study","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-19 01:31:48.374674","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Methanosarcina acetivorans protoglobin; Reductive nitrosylation offerric; Methanosarcina acetivorans protoglobin; Nitrosylation of ferrousMethanosarcina acetivorans protoglobin; Kinetics; Thermodynamics","OtherDescriptors":null,"Notes":null,"AnaPub":2013,"MonPub":null,"DateUpdate":"2022-05-11","DateCreate":"2016-10-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000315075200020","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.bbrc.2012.11.122"},"refs":null,"anarec":{"AnaID":280693,"PubliDate":2013,"Pagination":"1301-1305","XtraPublOfAnaID":null,"ISBN":null,"Volume":"430","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42249,"SerRR":"Biochemical and Biophysical Research Communications. 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