{"refrec":{"BRefID":280701,"RR":"<b>Verdoodt, F.; Willems, M.; Dhondt, I.; Houthoofd, W.; Bert, W.; De Vos, W.H.</b> (2012). Measurement of S-phase duration of adult stem cells in the flatworm <i>Macrostomum lignano</i> by double replication labelling and quantitative colocalization analysis. <i>Cell Biology International 36(12)</i>: 1251-1259. <a href=\"https://dx.doi.org/10.1042/CBI20120187\" target=\"_blank\">https://dx.doi.org/10.1042/CBI20120187</a>","BEntID":272720,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Cell Biology International 36(12)</i>: 1251-1259. <a href=\"https://dx.doi.org/10.1042/CBI20120187\" target=\"_blank\">https://dx.doi.org/10.1042/CBI20120187</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Verdoodt, F.; Willems, M.; Dhondt, I.; Houthoofd, W.; Bert, W.; De Vos, W.H.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Verdoodt, F. <i>et al.</i>","Englishabstract":"Platyhelminthes are highly attractive models for addressing fundamental aspects of stem cell biology <i>in vivo</i>. These organisms possess a unique stem cell system comprised of neoblasts that are the only proliferating cells during adulthood. We have investigated <i>T</i><sub>s</sub> (S-phase duration) of neoblasts during homoeostasis and regeneration in the flatworm, <i>Macrostomum lignano</i>. A double immunohistochemical technique was used, performing sequential pulses with the thymidine analogues CldU (chlorodeoxyuridine) and IdU (iododeoxyuridine), separated by variable chase times in the presence of colchicine. Owing to the localized nature of the fluorescent signals (cell nuclei) and variable levels of autofluorescence, standard intensity-based colocalization analyses could not be applied to accurately determine the colocalization. Therefore, an object-based colocalization approach was devised to score the relative number of double-positive cells. Using this approach, <i>T</i><sub>s</sub> (S-phase duration) in the main population of neoblasts was ∼13 h. During early regeneration, no significant change in <i>T</i><sub>s</sub> was observed.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Measurement of S-phase duration of adult stem cells in the flatworm <i>Macrostomum lignano</i> by double replication labelling and quantitative colocalization analysis","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"cell cycle; colocalization; neoblast; platyhelminthes; regeneration;S-phase","OtherDescriptors":null,"Notes":null,"AnaPub":2012,"MonPub":null,"DateUpdate":"2022-07-13","DateCreate":"2016-10-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000311144500019","VABBcode":null,"OpenAcc":0,"DOI":"10.1042/CBI20120187"},"refs":null,"anarec":{"AnaID":280701,"PubliDate":2012,"Pagination":"1251-1259","XtraPublOfAnaID":null,"ISBN":null,"Volume":"36","Issue":"12","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":265483,"SerRR":"Cell Biology International. 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