{"refrec":{"BRefID":280768,"RR":"<b>Chauvigné, F.; Tingaud-Sequeira, A.; Agulleiro, M.J.; Calusinska, M.; Gómez, A.; Finn, R.N.; Cerdà, J.</b> (2010). Functional and evolutionary analysis of flatfish gonadotropin receptors reveals cladal- and lineage-level divergence of the teleost glycoprotein receptor family. <i>Biol. Reprod. 82(6)</i>: 1088-1102. <a href=\"http://dx.doi.org/10.1095/biolreprod.109.082289\" target=\"_blank\">dx.doi.org/10.1095/biolreprod.109.082289</a>","BEntID":272787,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Biol. Reprod. 82(6)</i>: 1088-1102. <a href=\"https://dx.doi.org/10.1095/biolreprod.109.082289\" target=\"_blank\">https://dx.doi.org/10.1095/biolreprod.109.082289</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Chauvigné, F.; Tingaud-Sequeira, A.; Agulleiro, M.J.; Calusinska, M.; Gómez, A.; Finn, R.N.; Cerdà, J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Chauvigné, F. <i>et al.</i>","Englishabstract":"Pituitary gonadotropins, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) act via their cognate glycoprotein hormone receptors (GpHRs), FSH receptor (FSHR), and LH/choriogonadotropin receptor (LHCGR) to regulate gonad physiology. Here, we show that the flatfish Senegalese sole (<span class=\"named-content genus-species\">Solea senegalensis</span>) expresses functional isoforms of <i>fshr</i> and <i>lhcgr</i>, but the genomic origin, ligand activation, and tissue distribution of the receptor transcripts are more complex than expected. By integrating the molecular phylogeny of GpHRs with the syntenic loci of vertebrate orthologs, and by subsequently characterizing the physical maps with the phylogeny of flanking genes, we found that vertebrate GpHRs have undergone a divergent evolution. In Teleostei, <i>fshr</i> genes have a common descent and can be classified as <i>fshra</i>, whereas <i>lhcgrb</i> genes exist as alternatively coded genes even in closely related species. Structural analyses of the receptors revealed that Fshra has an elongated ligand-binding domain, containing an extra leucine-rich repeat that specifically arose in the Acanthomorpha because of exon duplication. Ectopic expression in <span class=\"named-content genus-species\">Xenopus laevis</span> oocytes demonstrated that sole Fshra responded to piscine Fsh and Lh, whereas Lhcgrba was preferentially activated by its cognate hormone. The expression pattern of sole <i>fshra</i> and <i>lhcgrba</i> in gonads during the reproductive cycle was consistent with earlier observations wherein Fshra regulates ovarian growth and spermatogenesis and Lhcgrb triggers gamete maturation, respectively. However, contrary to observations in other teleosts, <i>fshra</i> was localized exclusively in Sertoli cells of the testis, whereas <i>lhcgrba</i> was expressed in Leydig cells as well as in spermatids. These results demonstrate the presence of alternatively coded <i>lhcgr</i> isoforms (<i>lhcgrba</i> and <i>lhcgrbb</i>) in teleosts and suggest a role of the <i>lhcgrba</i> receptor in the differentiation of spermatids into spermatozoa in Senegalese sole.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Functional and evolutionary analysis of flatfish gonadotropin receptors reveals cladal- and lineage-level divergence of the teleost glycoprotein receptor family","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"evolution; follicle-stimulating hormone receptor; Fshr; gametogenesis;gene conversion; gonadotropin; Lhcgr; luteinizing hormone; ovary;spermatid; spermatogenesis; teleosts","OtherDescriptors":null,"Notes":null,"AnaPub":2010,"MonPub":null,"DateUpdate":"2016-10-19","DateCreate":"2016-10-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000277964500009","VABBcode":null,"OpenAcc":1,"DOI":"10.1095/biolreprod.109.082289"},"refs":null,"anarec":{"AnaID":280768,"PubliDate":2010,"Pagination":"1088-1102","XtraPublOfAnaID":null,"ISBN":null,"Volume":"82","Issue":"6","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42262,"SerRR":"Biology of Reproduction. 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