{"refrec":{"BRefID":280771,"RR":"<b>Piette, F.; D'Amico, S.; Struvay, C.; Mazzucchelli, G.; Renaut, J.; Tutino, M.L.; Danchin, A.; Leprince, P.; Feller, G.</b> (2010). Proteomics of life at low temperatures: trigger factor is the primary chaperone in the Antarctic bacterium <i>Pseudoalteromonas haloplanktis</i> TAC125. <i>Mol. Microbiol. 76(1)</i>: 120-132. <a href=\"http://dx.doi.org/10.1111/j.1365-2958.2010.07084.x\" target=\"_blank\">dx.doi.org/10.1111/j.1365-2958.2010.07084.x</a>","BEntID":272790,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Mol. Microbiol. 76(1)</i>: 120-132. <a href=\"https://dx.doi.org/10.1111/j.1365-2958.2010.07084.x\" target=\"_blank\">https://dx.doi.org/10.1111/j.1365-2958.2010.07084.x</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Piette, F.; D'Amico, S.; Struvay, C.; Mazzucchelli, G.; Renaut, J.; Tutino, M.L.; Danchin, A.; Leprince, P.; Feller, G.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Piette, F. <i>et al.</i>","Englishabstract":"The proteomes expressed at 4°C and 18°C by the psychrophilic Antarctic bacterium <i>Pseudoalteromonas haloplanktis</i> have been compared using two-dimensional differential in-gel electrophoresis, showing that translation, protein folding, membrane integrity and anti-oxidant activities are upregulated at 4°C. This proteomic analysis revealed that the trigger factor is the main upregulated protein at low temperature. The trigger factor is the first molecular chaperone interacting with virtually all newly synthesized polypeptides on the ribosome and also possesses a peptidyl-prolyl <i>cis-trans</i> isomerase activity. This suggests that protein folding at low temperatures is a rate-limiting step for bacterial growth in cold environments. It is proposed that the psychrophilic trigger factor rescues the chaperone function as both DnaK and GroEL (the major bacterial chaperones but also heat-shock proteins) are downregulated at 4°C. The recombinant psychrophilic trigger factor is a monomer that displays unusually low conformational stability with a <i>Tm</i> value of 33°C, suggesting that the essential chaperone function requires considerable flexibility and dynamics to compensate for the reduction of molecular motions at freezing temperatures. Its chaperone activity is strongly temperature-dependent and requires near-zero temperature to stably bind a model-unfolded polypeptide.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Proteomics of life at low temperatures: trigger factor is the primary chaperone in the Antarctic bacterium <i>Pseudoalteromonas haloplanktis</i> TAC125","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2010,"MonPub":null,"DateUpdate":"2016-10-20","DateCreate":"2016-10-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000276036000009","VABBcode":null,"OpenAcc":1,"DOI":"10.1111/j.1365-2958.2010.07084.x"},"refs":null,"anarec":{"AnaID":280771,"PubliDate":2010,"Pagination":"120-132","XtraPublOfAnaID":null,"ISBN":null,"Volume":"76","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":218506,"SerRR":"Molecular Microbiology. 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