{"refrec":{"BRefID":280778,"RR":"<b>Mikhailova, N.A.; Gracheva, Y.A.; Backeljau, T.; Granovitch, A.I.</b> (2009). A potential species-specific molecular marker suggests interspecific hybridization between sibling species <i>Littorina arcana</i> and <i>L. saxatilis</i> (Mollusca, Caenogastropoda) in natural populations. <i>Genetica 137(3)</i>: 333-340. <a href=\"https://dx.doi.org/10.1007/s10709-009-9397-4\" target=\"_blank\">https://dx.doi.org/10.1007/s10709-009-9397-4</a>","BEntID":272797,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Genetica 137(3)</i>: 333-340. <a href=\"https://dx.doi.org/10.1007/s10709-009-9397-4\" target=\"_blank\">https://dx.doi.org/10.1007/s10709-009-9397-4</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Mikhailova, N.A.; Gracheva, Y.A.; Backeljau, T.; Granovitch, A.I.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Mikhailova, N.A. <i>et al.</i>","Englishabstract":"Three sister species of rough periwinkles, viz. <i>Littorina saxatilis</i> (Olivi 1792), <i>L. arcana</i> (Hannaford Ellis 1978) and <i>L. compressa</i> (Jeffreys 1865) from the Barents Sea (Russia), the White Sea (Russia) and the Norwegian Sea (Norway) were studied. The identification of two sibling species <i>L. saxatilis</i> and <i>L. arcana</i> is often difficult as both species have extremely similar shell morphology and reproductive systems. Only mature females can be unambiguously distinguished, with a jelly gland present in female <i>L. arcana</i>, but which is replaced by a brood pouch containing developing embryos in <i>L. saxatilis</i>. No clear-cut diagnostic features have been found to discriminate between males or juveniles of the two species. The very first diagnostic DNA marker (DNA fragment A2.8, 271 bp length) for <i>L. arcana</i> and <i>L. saxatilis</i> separation was developed. The marker was derived from apparently species-specific <i>L. arcana</i> DNA fragments obtained via Random Amplified Polymorphic DNA (RAPD) analysis. This fragment was cloned and sequenced, whereupon specific primers were designed and the amplification was surveyed in a large number of morphologically well-identified females of both species. Subsequently, the specific DNA marker was used for the identification of male <i>L. arcana</i> and partners in copulating pairs. In this way, we obtained evidence of possible interspecific hybridization between the sibling species <i>L. arcana</i> and <i>L. saxatilis</i> living in sympatry in natural populations: the presence of A2.8 fragment in 12% of morphologically well identified <i>L. saxatilis</i> females and its absence in 14% of morphologically well identified <i>L. arcana</i> females. The A2.8 fragment never amplified in <i>L. saxatilis</i> from sites without <i>L. arcana</i>. The A2.8 fragment did not amplify in <i>L. compressa</i>, not even in microsympatric populations, and we did not observe interspecific copulations between <i>L. arcana</i> and <i>L. compressa</i>.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"A potential species-specific molecular marker suggests interspecific hybridization between sibling species <i>Littorina arcana</i> and <i>L. saxatilis</i> (Mollusca, Caenogastropoda) in natural populations","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:32:52.928458","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Littorina; RAPD cloned diagnostic marker; Male identification; Mating;Partner identification","OtherDescriptors":null,"Notes":null,"AnaPub":2009,"MonPub":null,"DateUpdate":"2022-05-03","DateCreate":"2016-10-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000271307300010","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s10709-009-9397-4"},"refs":null,"anarec":{"AnaID":280778,"PubliDate":2009,"Pagination":"333-340","XtraPublOfAnaID":null,"ISBN":null,"Volume":"137","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42731,"SerRR":"Genetica. Kluwer Academic: The Hague.  ISSN 0016-6707; e-ISSN 1573-6857","StandardTitleSer":"Genetica","ISSN":"0016-6707","AbbrevSer":"Genetica","StandardTitleMon":null,"StartPage":333,"Pages":8,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Mikhailova","Firstname":"Natalia","Initials":"N.A.","Affiliation":"Russian Acad Sci, Inst Cytol, St Petersburg 194064, Russia.","Discriminator":null,"CorporateFlag":0,"BEntID":272797,"AutID":241142,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Gracheva","Firstname":"Yulia","Initials":"Y.A.","Affiliation":"Russian Acad Sci, Inst Cytol, St Petersburg 194064, Russia.","Discriminator":null,"CorporateFlag":0,"BEntID":272797,"AutID":241143,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Backeljau","Firstname":"Thierry","Initials":"T.","Affiliation":"Royal Belgian Institute of Natural Sciences","Discriminator":null,"CorporateFlag":0,"BEntID":272797,"AutID":133586,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"IRScNB/KBIN","InsFSN":"Koninklijk Belgisch Instituut voor Natuurwetenschappen","ORCID":null,"PersID":271,"InsID":1883},{"AutName":"Granovitch","Firstname":"Andrey","Initials":"A.I.","Affiliation":"St Petersburg State Univ, St Petersburg, Russia.","Discriminator":null,"CorporateFlag":0,"BEntID":272797,"AutID":241147,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":376447,"LibID":36,"BRefID":280778,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"376447","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Kluwer Academic","City":"The Hague"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Littorina"}],"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":272797,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":272797,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1007/s10709-009-9397-4","externalID":"10.1007/s10709-009-9397-4","URLTypeCode":"DOI","URLID":51137,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Littorina","AphiaID":138135,"TaxtID":34477}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Littorina","DutchTerm":"Alikruiken","AphiaID":138135,"TaxtID":34477}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2016-10-09 21:07:26.660000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Chisala, Chilekwa, C.","updSesDate":{"date":"2022-05-03 11:08:02.403000","timezone_type":3,"timezone":"Europe/Brussels"}}}