{"refrec":{"BRefID":280786,"RR":"<b>Dolashka-Angelova, P.; Lieb, B.; Velkova, L.; Heilen, N.; Sandra, K.; Nikolaeva-Glomb, L.; Dolashki, A.; Galabov, A.S.; Van Beeumen, J.; Stevanovic, S.; Voelter, W.; Devreese, B.</b> (2009). Identification of glycosylated sites in <i>Rapana</i> hemocyanin by mass spectrometry and gene sequence, and their antiviral effect. <i>Bioconjugate Chemistry 20(7)</i>: 1315-1322. <a href=\"https://dx.doi.org/10.1021/bc900034k\" target=\"_blank\">https://dx.doi.org/10.1021/bc900034k</a>","BEntID":272805,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Bioconjugate Chemistry 20(7)</i>: 1315-1322. <a href=\"https://dx.doi.org/10.1021/bc900034k\" target=\"_blank\">https://dx.doi.org/10.1021/bc900034k</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Dolashka-Angelova, P.; Lieb, B.; Velkova, L.; Heilen, N.; Sandra, K.; Nikolaeva-Glomb, L.; Dolashki, A.; Galabov, A.S.; Van Beeumen, J.; Stevanovic, S.; Voelter, W.; Devreese, B.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Dolashka-Angelova, P. <i>et al.</i>","Englishabstract":"Molluscan hemocyanins (Hcs) have recently received particular interest due to their significant immunostimulatory properties. This is mainly related to their high carbohydrate content and specific monosaccharide composition. We have now analyzed the oligosaccharides and the carbohydrate linkage sites of the Rapana venosa hemocyanin (RvH) using different approaches. We analyzed a number of glycopeptides by LC/ESI-MS/MS and identified the sugar chains and peptide sequences of 12 glycopeptides. Additionally, the potential carbohydrate linkage sites of 2 functional units, RvH-b and RvH-c, were determined by gene sequence analysis. Only RvH-c shows a potential N-glycosylation site. During this study, we discovered a highly conserved linker-intron, separating the coding exons of RVH-b and RvH-c. Following reports on antiviral properties from arthropod hemocyanin, we conducted a preliminary study of the antiviral activity of RvH and the functional units RvH-b and RvH-c. We show that the glycosylated FU RvH-c has antiviral properties against the respiratory syncytial virus (RSV), whereas native RvH and the nonglycosylated FU RvH-b have not. This is the first report of the fact that also molluscan hemocyanin functional units possess antiviral activity.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Identification of glycosylated sites in <i>Rapana</i> hemocyanin by mass spectrometry and gene sequence, and their antiviral effect","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2009,"MonPub":null,"DateUpdate":"2022-07-13","DateCreate":"2016-10-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000268137700005","VABBcode":null,"OpenAcc":0,"DOI":"10.1021/bc900034k"},"refs":null,"anarec":{"AnaID":280786,"PubliDate":2009,"Pagination":"1315-1322","XtraPublOfAnaID":null,"ISBN":null,"Volume":"20","Issue":"7","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":264465,"SerRR":"Bioconjugate Chemistry. AMER CHEMICAL SOC: Washington.  ISSN 1043-1802","StandardTitleSer":"Bioconjugate Chemistry","ISSN":"1043-1802","AbbrevSer":null,"StandardTitleMon":null,"StartPage":1315,"Pages":8,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Dolashka-Angelova","Firstname":"Pavlina","Initials":"P.","Affiliation":"Bulgarian Acad Sci, Inst Organ Chem, Ctr Phytochem, BU-1113 Sofia, Bulgaria.","Discriminator":null,"CorporateFlag":0,"BEntID":272805,"AutID":241185,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Lieb","Firstname":"Bernhard","Initials":"B.","Affiliation":null,"Discriminator":null,"CorporateFlag":1,"BEntID":272805,"AutID":129720,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Velkova","Firstname":"Ludmila","Initials":"L.","Affiliation":"Bulgarian Acad Sci, Inst Organ Chem, Ctr Phytochem, BU-1113 Sofia, Bulgaria.","Discriminator":null,"CorporateFlag":0,"BEntID":272805,"AutID":241186,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Heilen","Firstname":"Nina","Initials":"N.","Affiliation":"Univ Tubingen, Inst Cell Biol, Dept Immunol, D-72076 Tubingen, Germany.","Discriminator":null,"CorporateFlag":0,"BEntID":272805,"AutID":241187,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Sandra","Firstname":"Koen","Initials":"K.","Affiliation":"Laboratory of Protein Biochemistry and Biomolecular Engineering, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":272805,"AutID":241188,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Biochemie, Fysiologie en Microbiologie; Laboratorium voor Eiwitbiochemie en Biomoleculaire engineering","ORCID":null,"PersID":null,"InsID":12157},{"AutName":"Nikolaeva-Glomb","Firstname":"Lubomira","Initials":"L.","Affiliation":"Bulgarian Acad Sci, Stephan Angeloff Inst Microbiol, BU-1113 Sofia, Bulgaria.","Discriminator":null,"CorporateFlag":0,"BEntID":272805,"AutID":241189,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Dolashki","Firstname":"Aleksander","Initials":"A.","Affiliation":"Univ Tubingen, Inst Biochem, D-72076 Tubingen, Germany.","Discriminator":null,"CorporateFlag":0,"BEntID":272805,"AutID":241190,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Galabov","Firstname":"Angel","Initials":"A.S.","Affiliation":"Bulgarian Acad Sci, Stephan Angeloff Inst Microbiol, BU-1113 Sofia, Bulgaria.","Discriminator":null,"CorporateFlag":0,"BEntID":272805,"AutID":241191,"OrderNr":8,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Van Beeumen","Firstname":"Jozef","Initials":"J.","Affiliation":"Laboratory of Protein Biochemistry and Biomolecular Engineering, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":272805,"AutID":243048,"OrderNr":9,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Biochemie, Fysiologie en Microbiologie; Laboratorium voor Eiwitbiochemie en Biomoleculaire engineering","ORCID":null,"PersID":603,"InsID":12157},{"AutName":"Stevanovic","Firstname":"Stefan","Initials":"S.","Affiliation":"Univ Tubingen, Inst Cell Biol, Dept Immunol, D-72076 Tubingen, Germany.","Discriminator":null,"CorporateFlag":0,"BEntID":272805,"AutID":241192,"OrderNr":10,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Voelter","Firstname":"Wolfgang","Initials":"W.","Affiliation":"Univ Tubingen, Inst Biochem, D-72076 Tubingen, Germany.","Discriminator":null,"CorporateFlag":0,"BEntID":272805,"AutID":241193,"OrderNr":11,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Devreese","Firstname":"Bart","Initials":"B.","Affiliation":"Laboratory of Protein Biochemistry and Biomolecular Engineering, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":272805,"AutID":243049,"OrderNr":12,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Biochemie, Fysiologie en Microbiologie; Laboratorium voor Eiwitbiochemie en Biomoleculaire engineering","ORCID":null,"PersID":null,"InsID":12157}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":379332,"LibID":36,"BRefID":280786,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"379332","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"AMER CHEMICAL SOC","City":"Washington"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":272805,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":272805,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1021/bc900034k","externalID":"10.1021/bc900034k","URLTypeCode":"DOI","URLID":51143,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2016-10-09 21:07:26.660000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"VLIZ2000\\elid","updSesDate":{"date":"2022-07-13 09:09:28.470000","timezone_type":3,"timezone":"Europe/Brussels"}}}