{"refrec":{"BRefID":280794,"RR":"<b>Delfosse, V.; Girard, E.; Birck, C.; Delmarcelle, M.; Delarue, M.; Poch, O.; Schultz, P.; Mayer, C.</b> (2009). Structure of the archaeal Pab87 peptidase reveals a novel self-compartmentalizing protease family. <i>PLoS One 4(3)</i>: e4712. <a href=\"http://dx.doi.org/10.1371/journal.pone.0004712\" target=\"_blank\">dx.doi.org/10.1371/journal.pone.0004712</a>","BEntID":272813,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>PLoS One 4(3)</i>: e4712. <a href=\"https://dx.doi.org/10.1371/journal.pone.0004712\" target=\"_blank\">https://dx.doi.org/10.1371/journal.pone.0004712</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Delfosse, V.; Girard, E.; Birck, C.; Delmarcelle, M.; Delarue, M.; Poch, O.; Schultz, P.; Mayer, C.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Delfosse, V. <i>et al.</i>","Englishabstract":"Self-compartmentalizing proteases orchestrate protein turnover through an original architecture characterized by a central catalytic chamber. Here we report the first structure of an archaeal member of a new self-compartmentalizing protease family forming a cubic-shaped octamer with <i>D</i><sub>4</sub> symmetry and referred to as CubicO. We solved the structure of the <i>Pyrococcus abyssi</i> Pab87 protein at 2.2 Å resolution using the anomalous signal of the high-phasing-power lanthanide derivative Lu-HPDO3A. A 20 Å wide channel runs through this supramolecular assembly of 0.4 MDa, giving access to a 60 Å wide central chamber holding the eight active sites. Surprisingly, activity assays revealed that Pab87 degrades specifically d-amino acid containing peptides, which have never been observed in archaea. Genomic context of the Pab87 gene showed that it is surrounded by genes involved in the amino acid/peptide transport or metabolism. We propose that CubicO proteases are involved in the processing of d-peptides from environmental origins.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Structure of the archaeal Pab87 peptidase reveals a novel self-compartmentalizing protease family","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2009,"MonPub":null,"DateUpdate":"2016-10-19","DateCreate":"2016-10-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000265490600022","VABBcode":null,"OpenAcc":1,"DOI":"10.1371/journal.pone.0004712"},"refs":null,"anarec":{"AnaID":280794,"PubliDate":2009,"Pagination":"e4712","XtraPublOfAnaID":null,"ISBN":null,"Volume":"4","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":123954,"SerRR":"PLoS One. 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