{"refrec":{"BRefID":280844,"RR":"<b>Ramser, K.; Wenseleers, W.; Dewilde, S.; Van Doorslaer, S.; Moens, L.; Hanstorp, D.</b> (2007). A combined micro-resonance Raman and absorption set-up enabling <i>in vivo</i> studies under varying physiological conditions: the nerve globin in the nerve cord of <i>Aphrodite aculeata</i>. <i>Journal of Biochemical and Biophysical Methods 70(4)</i>: 627-633. <a href=\"https://dx.doi.org/10.1016/j.jbbm.2007.02.003\" target=\"_blank\">https://dx.doi.org/10.1016/j.jbbm.2007.02.003</a>","BEntID":272863,"PublicFlag":1,"CheckedFlag":1,"wosflag":0,"vabbflag":0,"RefStringPartII":". <i>Journal of Biochemical and Biophysical Methods 70(4)</i>: 627-633. <a href=\"https://dx.doi.org/10.1016/j.jbbm.2007.02.003\" target=\"_blank\">https://dx.doi.org/10.1016/j.jbbm.2007.02.003</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Ramser, K.; Wenseleers, W.; Dewilde, S.; Van Doorslaer, S.; Moens, L.; Hanstorp, D.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Ramser, K. <i>et al.</i>","Englishabstract":"We hereby report on the design of a set-up combining micro-resonance Raman and absorption spectroscopy with a microfluidic system. The set-up enabled us to study the nerve globin of <i>Aphrodite aculeata</i> in the functional isolated nerve cord under varying physiological conditions for extended periods of time. The oxygenation cycle of the organism was triggered by utilizing the microfluidic system that allowed for a fast switch between aerobic and anaerobic conditions. The nerve globin was found to very easily shift from a penta-coordinated high spin ferrous form to the oxy state upon a change from anaerobic to aerobic conditions. The observed fast reaction to varying O<sub>2</sub> concentrations supports an oxygen-carrying and/or -storing function of the nerve globin. In addition, by combining resonance Raman and absorption spectroscopy, the physiological response could be distinguished from light-induced effects.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"A combined micro-resonance Raman and absorption set-up enabling <i>in vivo</i> studies under varying physiological conditions: the nerve globin in the nerve cord of <i>Aphrodite aculeata</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:32:52.928458","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"micro-resonance Raman spectroscopy; absorption spectroscopy;microfluidic system; nerve globin; Aphrodite aculeata","OtherDescriptors":null,"Notes":null,"AnaPub":2007,"MonPub":null,"DateUpdate":"2022-07-14","DateCreate":"2016-10-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000246933400013","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.jbbm.2007.02.003"},"refs":null,"anarec":{"AnaID":280844,"PubliDate":2007,"Pagination":"627-633","XtraPublOfAnaID":null,"ISBN":null,"Volume":"70","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":281894,"SerRR":"Journal of Biochemical and Biophysical Methods. 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