{"refrec":{"BRefID":281027,"RR":"<b>Violot, S.; Haser, R.; Sonan, G.; Georlette, D.; Feller, G.; Aghajari, N.</b> (2003). Expression, purification, crystallization and preliminary X-ray crystallographic studies of a psychrophilic cellulase from <i>Pseudoalteromonas haloplanktis</i>. <i>Acta Crystallographica Section D-Structural Biology 59</i>: 1256-1258. <a href=\"http://dx.doi.org/10.1107/S0907444903008849\" target=\"_blank\">dx.doi.org/10.1107/S0907444903008849</a>","BEntID":273046,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Acta Crystallogr. Sect. D 59</i>: 1256-1258. <a href=\"https://dx.doi.org/10.1107/S0907444903008849\" target=\"_blank\">https://dx.doi.org/10.1107/S0907444903008849</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Violot, S.; Haser, R.; Sonan, G.; Georlette, D.; Feller, G.; Aghajari, N.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Violot, S. <i>et al.</i>","Englishabstract":"The Antarctic psychrophile <i>Pseudoalteromonas haloplanktis</i> produces a cold-active cellulase. To date, a three-dimensional structure of a psychrophilic cellulase has been lacking. Crystallographic studies of this cold-adapted enzyme have therefore been initiated in order to contribute to the understanding of the molecular basis of the cold adaptation and the high catalytic efficiency of the enzyme at low and moderate temperatures. The catalytic core domain of the psychrophilic cellulase CelG from <i>P. haloplanktis</i> has been expressed, purified and crystallized and a complete diffraction data set to 1.8 Å has been collected. The space group was found to be <i>P</i>2<sub>1</sub>2<sub>1</sub>2<sub>1</sub>, with unit-cell parameters <i>a</i> = 135.1, <i>b</i> = 78.4, <i>c</i> = 44.1 Å. A molecular-replacement solution, using the structure of the mesophilic counterpart Cel5A from <i>Erwinia chrysanthemi</i> as a search model, has been found.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Expression, purification, crystallization and preliminary X-ray crystallographic studies of a psychrophilic cellulase from <i>Pseudoalteromonas haloplanktis</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"cellulase; extremophiles; psychrophilic enzymes","OtherDescriptors":null,"Notes":null,"AnaPub":2003,"MonPub":null,"DateUpdate":"2016-12-02","DateCreate":"2016-10-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000183809900025","VABBcode":null,"OpenAcc":0,"DOI":"10.1107/S0907444903008849"},"refs":null,"anarec":{"AnaID":281027,"PubliDate":2003,"Pagination":"1256-1258","XtraPublOfAnaID":null,"ISBN":null,"Volume":"59","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":262452,"SerRR":"Acta Crystallographica Section D-Structural Biology. International Union of Crystallography: Oxford.  ISSN 2059-7983","StandardTitleSer":"Acta Crystallographica Section D-Structural Biology","ISSN":"2059-7983","AbbrevSer":"Acta Crystallogr. Sect. 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