{"refrec":{"BRefID":281093,"RR":"<b>Tutino, M.L.; Duilio, A.; Parrilli, E.; Remaut, E.; Sannia, G.; Marino, G.</b> (2001). A novel replication element from an Antarctic plasmid as a tool for the expression of proteins at low temperature. <i>Extremophiles 5(4)</i>: 257-264. <a href=\"https://dx.doi.org/10.1007/s007920100203\" target=\"_blank\">https://dx.doi.org/10.1007/s007920100203</a>","BEntID":273112,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Extremophiles 5(4)</i>: 257-264. <a href=\"https://dx.doi.org/10.1007/s007920100203\" target=\"_blank\">https://dx.doi.org/10.1007/s007920100203</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Tutino, M.L.; Duilio, A.; Parrilli, E.; Remaut, E.; Sannia, G.; Marino, G.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Tutino, M.L. <i>et al.</i>","Englishabstract":"Genetic manipulation of Antarctic bacteria has been very limited so far. This article reports the isolation and molecular characterization of a novel plasmid, pMtBL, from the Antarctic gram-negative bacterium<i> Pseudoalteromonas haloplanktis</i> TAC 125. This genetic element, 4,081 bp long, appeared to be a multicopy cryptic replicon with no detectable transcriptional activity. By an in vivo assay, the pMtBL autonomous replication sequence was functionally limited to an<i> Alu</i>I plasmid fragment of about 850 bp. This novel cold-adapted replication element showed quite a broad host range profile; it was cloned into a mesophilic genetic construction, obtaining a cold-adapted expression vector that was able to promote the production of<i> P. haloplanktis</i> A23 α-amylase in a psychrophilic bacterium. 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