{"refrec":{"BRefID":281100,"RR":"<b>Dircksen, H.; Bocking, D.; Heyn, U.; Mandel, C.; Chung, J.S.; Baggerman, G.; Verhaert, P.; Daufeldt, S.; Plösch, T.; Jaros, P.P.; Waelkens, E.; Keller, R.; Webster, S.G.</b> (2001). Crustacean hyperglycaemic hormone (CHH)-like peptides and CHH-precursor-related peptides from pericardial organ neurosecretory cells in the shore crab, Carcinus maenas, are putatively spliced and modified products of multiple genes. <i>Biochem. J. 356(1)</i>: 159-170. <a href=\"https://dx.doi.org/10.1042/0264-6021:3560159\" target=\"_blank\">https://dx.doi.org/10.1042/0264-6021:3560159</a>","BEntID":273119,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Biochem. J. 356(1)</i>: 159-170. <a href=\"https://dx.doi.org/10.1042/0264-6021:3560159\" target=\"_blank\">https://dx.doi.org/10.1042/0264-6021:3560159</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Dircksen, H.; Bocking, D.; Heyn, U.; Mandel, C.; Chung, J.S.; Baggerman, G.; Verhaert, P.; Daufeldt, S.; Plösch, T.; Jaros, P.P.; Waelkens, E.; Keller, R.; Webster, S.G.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Dircksen, H. <i>et al.</i>","Englishabstract":"About 24 intrinsic neurosecretory neurons within the pericardial organs (POs) of the crab <i>Carcinus maenas</i> produce a novel crustacean hyperglycaemic hormone (CHH)-like peptide (PO-CHH) and two CHH-precursor-related peptides (PO-CPRP I and II) as identified immunochemically and by peptide chemistry. Edman sequencing and MS revealed PO-CHH as a 73 amino acid peptide (8630Da) with a free C-terminus. PO-CHH and sinus gland CHH (SG-CHH) share an identical N-terminal sequence, positions 1–40, but the remaining sequence, positions 41–73 or 41–72, differs considerably. PO-CHH may have different precursors, as cDNA cloning of PO-derived mRNAs has revealed several similar forms, one exactly encoding the peptide. All PO-CHH cDNAs contain a nucleotide stretch coding for the SG-CHH<sup>41–76</sup> sequence in the 3′-untranslated region (UTR). Cloning of crab testis genomic DNA revealed at least four CHH genes, the structure of which suggest that PO-CHH and SG-CHH arise by alternative splicing of precursors and possibly post-transcriptional modification of PO-CHH. The genes encode four exons, separated by three variable introns, encoding part of a signal peptide (exon I), the remaining signal peptide residues, a CPRP, the PO-CHH<sup>1–40</sup>/SG-CHH<sup>1–40</sup> sequences (exon II), the remaining PO-CHH residues (exon III) and the remaining SG-CHH residues and a 3′-UTR (exon IV). Precursor and gene structures are more closely related to those encoding related insect ion-transport peptides than to penaeid shrimp CHH genes. PO-CHH neither exhibits hyperglycaemic activity <i>in vivo</i>, nor does it inhibit Y-organ ecdysteroid synthesis <i>in vitro</i>. From the morphology of the neurons it seems likely that novel functions remain to be discovered.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Crustacean hyperglycaemic hormone (CHH)-like peptides and CHH-precursor-related peptides from pericardial organ neurosecretory cells in the shore crab, Carcinus maenas, are putatively spliced and modified products of multiple genes","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-19 01:31:48.609074","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"alternative splicing; immunocytochemistry; neuropeptide; neurosecretion","OtherDescriptors":null,"Notes":null,"AnaPub":2001,"MonPub":null,"DateUpdate":"2022-07-08","DateCreate":"2016-10-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000169056200019","VABBcode":null,"OpenAcc":0,"DOI":"10.1042/0264-6021:3560159"},"refs":null,"anarec":{"AnaID":281100,"PubliDate":2001,"Pagination":"159-170","XtraPublOfAnaID":null,"ISBN":null,"Volume":"356","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42250,"SerRR":"Biochemical journal. 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