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BAC-pool sequencing and analysis confirms growth-associated QTLs in the Asian seabass genome. <i>NPG Scientific Reports 6(36647)</i>: 13 pp. <a href=\"http://dx.doi.org/10.1038/srep36647\" target=\"_blank\">http://dx.doi.org/10.1038/srep36647</a>","BEntID":273595,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>NPG Scientific Reports 6(36647)</i>: 13 pp. <a href=\"http://dx.doi.org/10.1038/srep36647\" target=\"_blank\">http://dx.doi.org/10.1038/srep36647</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Shen, X.; Ngoh, S.Y.; Thevasagayam, N.M.; Prakki, S.R.S.; Bhandare, P.; Tan, A.W.K.; Tan, G.Q.; Singh, S.; Phua, N.C.H.; Vij, S.; Orbán, L.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Shen, X. <i>et al.</i>","Englishabstract":"The Asian seabass is an important marine food fish that has been cultured for several decades in Asia Pacific. However, the lack of a high quality reference genome has hampered efforts to improve its selective breeding. A 3D BAC pool set generated in this study was screened using 22 SSR markers located on linkage group 2 which contains a growth-related QTL region. Seventy-two clones corresponding to 22 FPC contigs were sequenced by Illumina MiSeq technology. We co-assembled the MiSeq-derived scaffolds from each FPC contig with error-corrected PacBio reads, resulting in 187 sequences covering 9.7 Mb. Eleven genes annotated within this region were found to be potentially associated with growth and their tissue-specific expression was investigated. Correlation analysis demonstrated that SNPs in ctsb, skp1 and ppp2ca can be potentially used as markers for selecting fast-growing fingerlings. Conserved syntenies between seabass LG2 and five other teleosts were identified. This study i) provided a 10 Mb targeted genome assembly; ii) demonstrated NGS of BAC pools as a potential approach for mining candidates underlying QTLs of this species; iii) detected eleven genes potentially responsible for growth in the QTL region; and iv) identified useful SNP markers for selective breeding programs of Asian seabass.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"BAC-pool sequencing and analysis confirms growth-associated QTLs in the Asian seabass genome","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-15 01:32:57.644691","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2016,"MonPub":null,"DateUpdate":"2018-02-13","DateCreate":"2016-11-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000392058200001","VABBcode":null,"OpenAcc":1,"DOI":"10.1038/srep36647"},"refs":null,"anarec":{"AnaID":281576,"PubliDate":2016,"Pagination":"13 pp","XtraPublOfAnaID":null,"ISBN":null,"Volume":"6","Issue":"36647","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":208093,"SerRR":"Scientific Reports (Nature Publishing Group). 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