{"refrec":{"BRefID":281589,"RR":"<b>Laso-Pérez, R.; Wegener, G.; Knittel, K.; Widdel, F.; Harding, K.J.; Krukenberg, V.; Meier, D.V.; Richter, M.; Tegetmeyer, H.E.; Riedel, D.; Richnow, H.-H.; Adrian, L.; Reemtsma, T.; Lechtenfeld, O.J.; Musat, F.</b> (2016). Thermophilic archaea activate butane via alkyl-coenzyme M formation. <i>Nature (Lond.) 539(7629)</i>: 396-401. <a href=\"http://dx.doi.org/10.1038/nature20152\" target=\"_blank\">http://dx.doi.org/10.1038/nature20152</a>","BEntID":273608,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Nature (Lond.) 539(7629)</i>: 396-401. <a href=\"http://dx.doi.org/10.1038/nature20152\" target=\"_blank\">http://dx.doi.org/10.1038/nature20152</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Laso-Pérez, R.; Wegener, G.; Knittel, K.; Widdel, F.; Harding, K.J.; Krukenberg, V.; Meier, D.V.; Richter, M.; Tegetmeyer, H.E.; Riedel, D.; Richnow, H.-H.; Adrian, L.; Reemtsma, T.; Lechtenfeld, O.J.; Musat, F.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Laso-Pérez, R. <i>et al.</i>","Englishabstract":"The anaerobic formation and oxidation of methane involve unique enzymatic mechanisms and cofactors, all of which are believed to be specific for C-1-compounds. Here we show that an anaerobic thermophilic enrichment culture composed of dense consortia of archaea and bacteria apparently uses partly similar pathways to oxidize the C-4 hydrocarbon butane. The archaea, proposed genus Candidatus Syntrophoarchaeum', show the characteristic autofluorescence of methanogens, and contain highly expressed genes encoding enzymes similar to methyl-coenzyme M reductase. We detect butyl-coenzyme M, indicating archaeal butane activation analogous to the first step in anaerobic methane oxidation. In addition, Ca. Syntrophoarchaeum expresses the genes encoding beta-oxidation enzymes, carbon monoxide dehydrogenase and reversible C-1 methanogenesis enzymes. This allows for the complete oxidation of butane. Reducing equivalents are seemingly channelled to HotSeep-1, a thermophilic sulfate-reducing partner bacterium known from the anaerobic oxidation of methane. Genes encoding 16S rRNA and methyl-coenzyme M reductase similar to those identifying Ca. Syntrophoarchaeum were repeatedly retrieved from marine subsurface sediments, suggesting that the presented activation mechanism is naturally widespread in the anaerobic oxidation of short-chain hydrocarbons.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Thermophilic archaea activate butane via alkyl-coenzyme M formation","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-10 01:32:04.257239","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2016,"MonPub":null,"DateUpdate":"2018-02-13","DateCreate":"2016-11-18","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000388161700047","VABBcode":null,"OpenAcc":0,"DOI":"10.1038/nature20152"},"refs":null,"anarec":{"AnaID":281589,"PubliDate":2016,"Pagination":"396-401","XtraPublOfAnaID":null,"ISBN":null,"Volume":"539","Issue":"7629","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43431,"SerRR":"Nature: International Weekly Journal of Science. 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