{"refrec":{"BRefID":282121,"RR":"<b>Roosa, S.; Vander Wauven, C.; Billon, G.; Matthijs, S.; Wattiez, R.; Gillan, D.C.</b> (2014). The <i>Pseudomonas</i> community in metal-contaminated sediments as revealed by quantitative PCR: a link with metal bioavailability. <i>Research in Microbiology 165(8)</i>: 647-656. <a href=\"http://dx.doi.org/10.1016/j.resmic.2014.07.011\" target=\"_blank\">http://dx.doi.org/10.1016/j.resmic.2014.07.011</a>","BEntID":274140,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Research in Microbiology 165(8)</i>: 647-656. <a href=\"http://dx.doi.org/10.1016/j.resmic.2014.07.011\" target=\"_blank\">http://dx.doi.org/10.1016/j.resmic.2014.07.011</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Roosa, S.; Vander Wauven, C.; Billon, G.; Matthijs, S.; Wattiez, R.; Gillan, D.C.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Roosa, S. <i>et al.</i>","Englishabstract":"<i>Pseudomonas</i> bacteria are ubiquitous Gram-negative and aerobic microorganisms that are known to harbor metal resistance mechanisms such as efflux pumps and intracellular redox enzymes. Specific <i>Pseudomonas</i> bacteria have been quantified in some metal-contaminated environments, but the entire <i>Pseudomonas</i> population has been poorly investigated under these conditions, and the link with metal bioavailability was not previously examined. In the present study, quantitative PCR and cell cultivation were used to monitor and characterize the <i>Pseudomonas</i> population at 4 different sediment sites contaminated with various levels of metals. At the same time, total metals and metal bioavailability (as estimated using an HCl 1 m extraction) were measured. It was found that the total level of <i>Pseudomonas</i>, as determined by qPCR using two different genes (<i>oprI</i> and the 16S rRNA gene), was positively and significantly correlated with total and HCl-extractable Cu, Co, Ni, Pb and Zn, with high correlation coefficients (>0.8). Metal-contaminated sediments featured isolates of the <i>Pseudomonas putida</i>, <i>Pseudomonas fluorescens</i>, <i>Pseudomonas lutea</i> and <i>Pseudomonas aeruginosa</i> groups, with other bacterial genera such as <i>Mycobacterium</i>, <i>Klebsiella</i> and <i>Methylobacterium</i>. It is concluded that <i>Pseudomonas</i> bacteria do proliferate in metal-contaminated sediments, but are still part of a complex community.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"The <i>Pseudomonas</i> community in metal-contaminated sediments as revealed by quantitative PCR: a link with metal bioavailability","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"    Pseudomonas; Sediments; Metals; Bioavailability; Resistance; Quantitative PCR","OtherDescriptors":null,"Notes":null,"AnaPub":2014,"MonPub":null,"DateUpdate":"2018-05-17","DateCreate":"2016-12-22","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000347675700006","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.resmic.2014.07.011"},"refs":null,"anarec":{"AnaID":282121,"PubliDate":2014,"Pagination":"647-656","XtraPublOfAnaID":null,"ISBN":null,"Volume":"165","Issue":"8","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":277357,"SerRR":"Research in Microbiology. 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