{"refrec":{"BRefID":282256,"RR":"<b>Ardura, A.; Zaiko, A.; Martinez, J.L.; Samulioviene, A.; Semenova, A.; Garcia-Vazquez, E.</b> (2015). eDNA and specific primers for early detection of invasive species – A case study on the bivalve <i>Rangia cuneata</i>, currently spreading in Europe. <i>Mar. Environ. Res. 112(Part. B)</i>: 48-55. <a href=\"https://dx.doi.org/10.1016/j.marenvres.2015.09.013\" target=\"_blank\">https://dx.doi.org/10.1016/j.marenvres.2015.09.013</a>","BEntID":274275,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Mar. Environ. Res. 112(Part. B)</i>: 48-55. <a href=\"https://dx.doi.org/10.1016/j.marenvres.2015.09.013\" target=\"_blank\">https://dx.doi.org/10.1016/j.marenvres.2015.09.013</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Ardura, A.; Zaiko, A.; Martinez, J.L.; Samulioviene, A.; Semenova, A.; Garcia-Vazquez, E.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Ardura, A. <i>et al.</i>","Englishabstract":"Intense human activities facilitate the successful spread and establishment of non-indigenous aquatic organisms in marine and freshwater ecosystems. In some cases such intrusions result in noticeable and adverse changes in the recipient environments. In the Baltic Sea, the discovery and rapid initial spread of the North American wedge clam <i>Rangia cuneata</i> represents a new wave of invasion which may trigger unpredictable changes of the local benthic communities. In this study we present a species-specific DNA-based marker developed <i>in silico</i> and experimentally tested on environmental samples. Marker specificity and sensitivity were assessed <i>in vitro</i> from water samples containing different mixtures of the target species and other five bivalves currently present in the region: the native <i>Cerastoderma glaucum</i>, <i>Macoma balthica</i> and <i>Mytilus trossulus</i>, the invasive <i>Dreissena polymorpha</i> and the cryptogenic <i>Mya arenaria</i>. Cross-species amplification was not found in any case. The method allows to detecting at least 0.4 ng of <i>R. cuneata</i> DNA per μl, and 0.1 g of tissue per liter of water. Finally, the marker performance was assessed in water samples from the Baltic Sea and Vistula Lagoon. The coincidence between independent visual observations of <i>R. cuneata</i> and positive PCR amplification of the marker from the water samples confirmed the efficiency of this highly reproducible, fast, and technically easy method. <i>R. cuneata</i> traces can be detected from environmental DNA even when the population is sparse and small, enabling rapid management responses and allowing to track the invasion dynamics.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"eDNA and specific primers for early detection of invasive species – A case study on the bivalve <i>Rangia cuneata</i>, currently spreading in Europe","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-18 01:31:57.003804","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"    Baltic Sea; Non-indigenous species; Species-specific marker; Rangia cuneata; eDNA; Vistula Lagoon","OtherDescriptors":null,"Notes":null,"AnaPub":2015,"MonPub":null,"DateUpdate":"2019-09-30","DateCreate":"2017-01-05","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000367021700007","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.marenvres.2015.09.013"},"refs":null,"anarec":{"AnaID":282256,"PubliDate":2015,"Pagination":"48-55","XtraPublOfAnaID":null,"ISBN":null,"Volume":"112","Issue":"Part. B","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43357,"SerRR":"Marine Environmental Research. Applied Science Publishers: Barking.  ISSN 0141-1136; e-ISSN 1879-0291","StandardTitleSer":"Marine Environmental Research","ISSN":"0141-1136","AbbrevSer":"Mar. Environ. Res.","StandardTitleMon":null,"StartPage":48,"Pages":8,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Ardura","Firstname":"Alba","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":274275,"AutID":246560,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Zaiko","Firstname":"Anastasija","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":274275,"AutID":67186,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Martinez","Firstname":"Jose","Initials":"J.L.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":274275,"AutID":51802,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Samulioviene","Firstname":"Aurelija","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":274275,"AutID":246561,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Semenova","Firstname":"Anna","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":274275,"AutID":246562,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Garcia-Vazquez","Firstname":"Eva","Initials":"E.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":274275,"AutID":51803,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":10539,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":297809,"LibID":36,"BRefID":282256,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"297809","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Applied Science Publishers","City":"Barking"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Rangia cuneata"}],"geotermsASFA":null,"collections":[{"Collection":"VLIZ Acknowledged Publications","ShortName":"VLIZ ackn"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"941":{"SpName":"LifeWatch Species Information Backbone","SpColID":941,"ParSpColID":39,"TopParID":39,"ShortName":"LifeWatch Species Information Backbone","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":39,"SpColPath":"VLIZ ackn/LifeWatch Species Information Backbone"},"39":{"SpName":"VLIZ Acknowledged Publications","SpColID":39,"ParSpColID":null,"TopParID":null,"ShortName":"VLIZ ackn","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"VLIZ ackn"},"507":{"SpName":"World Register of Marine Species","SpColID":507,"ParSpColID":null,"TopParID":null,"ShortName":"WoRMS website","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"WoRMS website"},"915":{"SpName":"World Register of Marine Species (WoRMS) acknowledged","SpColID":915,"ParSpColID":941,"TopParID":39,"ShortName":"WoRMS ackn","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":39,"SpColPath":"VLIZ ackn/LifeWatch Species Information Backbone/WoRMS ackn"},"947":{"SpName":"WoRMS ackn - direct reference","SpColID":947,"ParSpColID":915,"TopParID":39,"ShortName":"WoRMS ackn - direct","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":39,"SpColPath":"VLIZ ackn/LifeWatch Species Information Backbone/WoRMS ackn/WoRMS ackn - direct"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":274275,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":274275,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/j.marenvres.2015.09.013","externalID":"10.1016/j.marenvres.2015.09.013","URLTypeCode":"DOI","URLID":78886,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Rangia cuneata","AphiaID":156991,"TaxtID":35707}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Rangia cuneata","DutchTerm":"Amerikaanse strandschelp","AphiaID":156991,"TaxtID":35707}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Bouchti, Zohra, Z.","newSesDate":{"date":"2017-01-05 09:52:10.247000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Chisala, Chilekwa, C.","updSesDate":{"date":"2019-09-30 10:07:04.967000","timezone_type":3,"timezone":"Europe/Brussels"}}}