{"refrec":{"BRefID":285297,"RR":"<b>Iryani, M.; MacRae, H.; Panchakshari, S.; Tan, B.; Bossier, P; Abd Wahid, E.; Sung, Y.</b> (2017). Knockdown of heat shock protein 70 (Hsp70) by RNAi reduces the tolerance of <i>Artemia franciscana</i> nauplii to heat and bacterial infection. <i>J. Exp. Mar. Biol. Ecol. 487</i>: 106-112. <a href=\"https://dx.doi.org/10.1016/j.jembe.2016.12.004\" target=\"_blank\">https://dx.doi.org/10.1016/j.jembe.2016.12.004</a>","BEntID":277322,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>J. Exp. Mar. Biol. Ecol. 487</i>: 106-112. <a href=\"https://dx.doi.org/10.1016/j.jembe.2016.12.004\" target=\"_blank\">https://dx.doi.org/10.1016/j.jembe.2016.12.004</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Iryani, M.; MacRae, H.; Panchakshari, S.; Tan, B.; Bossier, P; Abd Wahid, E.; Sung, Y.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Iryani, M. <i>et al.</i>","Englishabstract":"RNA interference (RNAi) was used to assess the role of the molecular chaperone Hsp70 in protecting nauplii of the crustacean, Artemia franciscana, against abiotic and biotic stressors. As revealed respectively by RT-PCR and immunoprobing of Western blots, the injection of A. franciscana females with Hsp70 dsRNA eliminated Hsp70 mRNA and protein in nauplii released from these animals. The loss of Hsp70 had no apparent effect on either embryo development or the viability of A. franciscana nauplii. The survival of nauplii lacking Hsp70, as compared to those containing the Hsp, was reduced 41% by heat stress and 34% upon Vibrio campbellii infection. A role for Hsp70 in protecting A. franciscana nauplii against heat stress and bacterial infection was demonstrated for the first time by using RNAi to knock down the protein. The results suggest that Hsp70 assisted in maintaining protein homeostasis within stressed cells while potentially stimulating the innate immune system of A. franciscana. Information of the type generated in this work has potential for application in the culture of A. franciscana and other commercially important aquatic invertebrates.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Knockdown of heat shock protein 70 (Hsp70) by RNAi reduces the tolerance of <i>Artemia franciscana</i> nauplii to heat and bacterial infection","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-17 01:32:13.054413","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Hsp70; Artemia franciscana; Nauplii; RNA interference (RNAi);Thermotolerance; Vibrio campbellii","OtherDescriptors":null,"Notes":null,"AnaPub":2017,"MonPub":null,"DateUpdate":"2019-08-06","DateCreate":"2017-05-16","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000392999900012","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.jembe.2016.12.004"},"refs":null,"anarec":{"AnaID":285297,"PubliDate":2017,"Pagination":"106-112","XtraPublOfAnaID":null,"ISBN":null,"Volume":"487","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43229,"SerRR":"Journal of Experimental Marine Biology and Ecology. Elsevier: New York.  ISSN 0022-0981; e-ISSN 1879-1697","StandardTitleSer":"Journal of Experimental Marine Biology and Ecology","ISSN":"0022-0981","AbbrevSer":"J. Exp. Mar. Biol. Ecol.","StandardTitleMon":null,"StartPage":106,"Pages":7,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Iryani","Firstname":"MTM","Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":277322,"AutID":255431,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"MacRae","Firstname":"TH","Initials":"H.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":277322,"AutID":255432,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Panchakshari","Firstname":"S","Initials":"S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":277322,"AutID":255433,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Tan","Firstname":"JB","Initials":"B.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":277322,"AutID":255434,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Bossier","Firstname":"Peter","Initials":"P","Affiliation":"Laboratory of Aquaculture and Artemia Reference Center, Department of Animal production, University of Ghent","Discriminator":null,"CorporateFlag":0,"BEntID":277322,"AutID":207336,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ARC","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Aquacultuur en Artemia Reference Center","ORCID":"0000-0002-6165-9111","PersID":125,"InsID":22},{"AutName":"Abd Wahid","Firstname":"ME","Initials":"E.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":277322,"AutID":255435,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Sung","Firstname":"YY","Initials":"Y.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":277322,"AutID":255436,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":332536,"LibID":36,"BRefID":285297,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"332536","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier","City":"New York"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Artemia franciscana"},{"TaxTerm":"Vibrio campbellii"}],"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":277322,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":277322,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/j.jembe.2016.12.004","externalID":"10.1016/j.jembe.2016.12.004","URLTypeCode":"DOI","URLID":55031,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Artemia franciscana","AphiaID":717572,"TaxtID":22672},{"TaxTerm":"Vibrio campbellii","AphiaID":570767,"TaxtID":35639}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Artemia franciscana","DutchTerm":null,"AphiaID":717572,"TaxtID":22672},{"TaxTerm":"Vibrio campbellii","DutchTerm":null,"AphiaID":570767,"TaxtID":35639}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2017-05-16 19:42:31.320000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Chisala, Chilekwa, C.","updSesDate":{"date":"2019-08-06 07:54:04.280000","timezone_type":3,"timezone":"Europe/Brussels"}}}