{"refrec":{"BRefID":285412,"RR":"<b>Sui, L.; Ma, G.; Lu, W.L.; Deng, Y.; Bossier, P.; De Schryver, P.</b> (2016). Effect of poly-β-hydroxybutyrate on growth, enzyme activity and intestinal microbial community of Chinese mitten crab, <i>Eriocheir sinensis</i> (Milne-Edwards) juveniles. <i>Aquac. Res. 47(11)</i>: 3644-3652. <a href=\"https://dx.doi.org/10.1111/are.12817\" target=\"_blank\">https://dx.doi.org/10.1111/are.12817</a>","BEntID":277437,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Aquac. Res. 47(11)</i>: 3644-3652. <a href=\"https://dx.doi.org/10.1111/are.12817\" target=\"_blank\">https://dx.doi.org/10.1111/are.12817</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Sui, L.; Ma, G.; Lu, W.L.; Deng, Y.; Bossier, P.; De Schryver, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Sui, L. <i>et al.</i>","Englishabstract":"Poly-β-hydroxybutyrate (PHB) is microbial carbon and energy storage polymer, which can be degraded into water-soluble β-hydroxybutyric acid in the gastrointestinal tract of aquatic animals. A 60-day culture experiment was performed using Chinese mitten crab, <i>Eriocheir sinensis</i> (Milne-Edwards) juveniles with an average initial body weight of 0.74 ± 0.06 g which were fed a diet supplemented with 0%, 0.5%, 1%, 3% or 5% PHB. A PHB dietary supplementation of 1% and 3% significantly improved the body weight gain, moulting frequency and concomitantly reduced 2nd–3rd moulting intervals of the crabs (<i>P</i> < 0.05). The dietary PHB level positively related to hepatopancreatic pepsin, trypsin and lipase activity (<i>P</i> < 0.05). Increasing the dietary PHB also improved total superoxide dismutase activity, but reduced alkaline phosphatase and acid phosphatase activity in the serum of hemolymph (<i>P</i> < 0.05). A 16S rRNA gene analysis by denaturing gradient gel electrophoresis indicated that PHB supplementation led to a significantly higher range-weighted richness, diversity and evenness of the gut bacterial community when dosed at 3% in the feed. 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