{"refrec":{"BRefID":285536,"RR":"<b>Das, K.; Dupont, A.; De Pauw-Gillet, M.-C.; Debier, C.; Siebert, U.</b> (2016). Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes <i>in vitro</i>. <i>Mar. Pollut. Bull. 108(1-2)</i>: 70-76. <a href=\"https://dx.doi.org/10.1016/j.marpolbul.2016.04.060\" target=\"_blank\">https://dx.doi.org/10.1016/j.marpolbul.2016.04.060</a>","BEntID":277561,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Mar. Pollut. Bull. 108(1-2)</i>: 70-76. <a href=\"https://dx.doi.org/10.1016/j.marpolbul.2016.04.060\" target=\"_blank\">https://dx.doi.org/10.1016/j.marpolbul.2016.04.060</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Das, K.; Dupont, A.; De Pauw-Gillet, M.-C.; Debier, C.; Siebert, U.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Das, K. <i>et al.</i>","Englishabstract":"Previous studies described high concentrations of mercury (Hg) and selenium (Se) in the blood of harbour seals, <i>Phoca vitulina</i> from the North Sea. In the present study, we evaluated the <i>in vitro</i> potential protective effects of sodium selenite (Na<sub>2</sub>SeO<sub>3</sub>) and selenomethionine (SeMet) on cell proliferation of harbour seal lymphocytes exposed to MeHgCl 0.75 μM. <i>In vitro</i> exposure of ConA-stimulated T lymphocytes resulted in severe inhibition of DNA synthesis, likely linked to severe loss of mitochondrial membrane potential at 0.75 μM. Neither selenite nor SeMet showed a protective effect against MeHg toxicity expressed at the T lymphocyte proliferation level for harbour seals. Selenite and SeMet did not show negative effects regarding lymphocyte proliferation and mitochondrial membrane potential.To conclude, our results clearly demonstrated that MeHg affected <i>in vitro</i> immune cells exposure with no protective effects of selenium at a molar ratio Hg:Se of 1:10 in harbour seals from the North Sea.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes <i>in vitro</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-17 01:32:13.523203","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Marine mammals; Harbour seal; Phoca vitulina; Methylmercury; Selenium;Lymphocytes; In vitro approach","OtherDescriptors":null,"Notes":null,"AnaPub":2016,"MonPub":null,"DateUpdate":"2017-05-19","DateCreate":"2017-05-16","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000379102900022","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.marpolbul.2016.04.060"},"refs":null,"anarec":{"AnaID":285536,"PubliDate":2016,"Pagination":"70-76","XtraPublOfAnaID":null,"ISBN":null,"Volume":"108","Issue":"1-2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43520,"SerRR":"Marine Pollution Bulletin. 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