{"refrec":{"BRefID":285572,"RR":"<b>Buerger, P.; Alvarez-Roa, C.; Weynberg, K.D.; Baekelandt, S.; van Oppen, M.J.H.</b> (2016). Genetic, morphological and growth characterisation of a new <i>Roseofilum</i> strain (Oscillatoriales, Cyanobacteria associated with coral black band disease. <i>PeerJ 4</i>: 19 pp. <a href=\"https://dx.doi.org/10.7717/peerj.2110\" target=\"_blank\">https://dx.doi.org/10.7717/peerj.2110</a>","BEntID":277597,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>PeerJ 4</i>: 19 pp. <a href=\"https://dx.doi.org/10.7717/peerj.2110\" target=\"_blank\">https://dx.doi.org/10.7717/peerj.2110</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Buerger, P.; Alvarez-Roa, C.; Weynberg, K.D.; Baekelandt, S.; van Oppen, M.J.H.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Buerger, P. <i>et al.</i>","Englishabstract":"Black band disease (BBD) is a common disease of reef-building corals with a worldwide distribution that causes tissue loss at a rate of up to 3 cm/day. Critical for a mechanistic understanding of the disease’s aetiology is the cultivation of its proposed pathogen, filamentous cyanobacteria (genus <i>Roseofilum</i>). Here, we optimise existing protocols for the isolation and cultivation of <i>Roseofilum</i> cyanobacteria using a new strain from the central Great Barrier Reef. We demonstrate that the isolation of this bacterium <i>via</i> inoculation onto agar plates was highly effective with a low percentage agar of 0.6% and that growth monitoring was most sensitive with fluorescence measurements of chlorophyll-a (440/685 nm). Cell growth curves in liquid and solid media were generated for the first time for this cyanobacterium and showed best growth rates for the previously untested L1-medium (growth rate <i>k</i> = 0.214 biomass/day; doubling time <i>t</i><sub>gen</sub> = 4.67 days). Our results suggest that the trace metals contained in L1-medium maximise biomass increase over time for this cyanobacterium. Since the newly isolated <i>Roseofilum</i> strain is genetically closest to <i>Pseudoscillatoria coralii</i>, but in terms of pigmentation and cell size closer to <i>Roseofilum</i>  <i>reptotaenium</i>, we formally merge the two species into a single taxon by providing an emended species description, <i>Roseofilum reptotaenium</i> (Rasoulouniriana) Casamatta emend. Following this optimized protocol is recommended for fast isolation and cultivation of <i>Roseofilum</i> cyanobacteria, for growth curve generation in strain comparisons and for maximisation of biomass in genetic studies.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Genetic, morphological and growth characterisation of a new <i>Roseofilum</i> strain (Oscillatoriales, Cyanobacteria associated with coral black band disease","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Cultivation; Coral disease; BBD; Cyanobacteria isolation; Microbial mat;Roseofillum reptotaenium; Pseudoscillatoria coralii; Great Barrier Reef;Australia","OtherDescriptors":null,"Notes":null,"AnaPub":2016,"MonPub":null,"DateUpdate":"2017-05-22","DateCreate":"2017-05-16","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000378027200006","VABBcode":null,"OpenAcc":1,"DOI":"10.7717/peerj.2110"},"refs":null,"anarec":{"AnaID":285572,"PubliDate":2016,"Pagination":"19 pp","XtraPublOfAnaID":null,"ISBN":null,"Volume":"4","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":242055,"SerRR":"PeerJ. 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