{"refrec":{"BRefID":286148,"RR":"<b>Vergucht, E.; Brans, T.; Beunis, F.; Garrevoet, J.; De Rijcke, M.; Bauters, S.; Vandegehuchte, M.; Van Nieuwenhove, I.; Janssen, C.; Burghammer, M.; Vincze, L.</b> (2015). <i>In vivo</i> X-ray elemental imaging of single cell model organisms manipulated by laser-based optical tweezers. <i>NPG Scientific Reports 5</i>: 9049. <a href=\"https://dx.doi.org/10.1038/srep09049\" target=\"_blank\">https://dx.doi.org/10.1038/srep09049</a>","BEntID":278170,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>NPG Scientific Reports 5</i>: 9049. <a href=\"https://dx.doi.org/10.1038/srep09049\" target=\"_blank\">https://dx.doi.org/10.1038/srep09049</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Vergucht, E.; Brans, T.; Beunis, F.; Garrevoet, J.; De Rijcke, M.; Bauters, S.; Vandegehuchte, M.; Van Nieuwenhove, I.; Janssen, C.; Burghammer, M.; Vincze, L.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Vergucht, E. <i>et al.</i>","Englishabstract":"We report on a radically new elemental imaging approach for the analysis of biological model organisms and single cells in their natural, <i>in vivo</i> state. The methodology combines optical tweezers (OT) technology for non-contact, laser-based sample manipulation with synchrotron radiation confocal X-ray fluorescence (XRF) microimaging for the first time. The main objective of this work is to establish a new method for <i>in vivo</i> elemental imaging in a two-dimensional (2D) projection mode in free-standing biological microorganisms or single cells, present in their aqueous environment. Using the model organism <i>Scrippsiella trochoidea</i>, a first proof of principle experiment at beamline ID13 of the European Synchrotron Radiation Facility (ESRF) demonstrates the feasibility of the OT XRF methodology, which is applied to study mixture toxicity of Cu-Ni and Cu-Zn as a result of elevated exposure. We expect that the new OT XRF methodology will significantly contribute to the new trend of investigating microorganisms at the cellular level with added <i>in vivo</i> capability.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"<i>In vivo</i> X-ray elemental imaging of single cell model organisms manipulated by laser-based optical tweezers","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-20 01:32:19.998020","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2015,"MonPub":null,"DateUpdate":"2018-03-08","DateCreate":"2017-06-08","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000351144900026","VABBcode":null,"OpenAcc":1,"DOI":"10.1038/srep09049"},"refs":null,"anarec":{"AnaID":286148,"PubliDate":2015,"Pagination":"9049","XtraPublOfAnaID":null,"ISBN":null,"Volume":"5","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":208093,"SerRR":"Scientific Reports (Nature Publishing Group). Nature Publishing Group: London.  ISSN 2045-2322; e-ISSN 2045-2322","StandardTitleSer":"Scientific Reports (Nature Publishing Group)","ISSN":"2045-2322","AbbrevSer":"NPG Scientific Reports","StandardTitleMon":null,"StartPage":9049,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Vergucht","Firstname":"Eva","Initials":"E.","Affiliation":"X-ray Microspectroscopy and Imaging Group, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":278170,"AutID":259242,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"XMI","InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Analytische Chemie; X-stralen Microspectroscopie en Beeldvorming","ORCID":null,"PersID":35194,"InsID":14069},{"AutName":"Brans","Firstname":"Toon","Initials":"T.","Affiliation":"Department of Electronics and Information Systems, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":278170,"AutID":259243,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ELIS","InsFSN":"Ghent University; Faculty of Engineering; Department of Electronics and information systems","ORCID":null,"PersID":35762,"InsID":2247},{"AutName":"Beunis","Firstname":"Filip","Initials":"F.","Affiliation":"Department of Electronics and Information Systems, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":278170,"AutID":259245,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ELIS","InsFSN":"Ghent University; Faculty of Engineering; Department of Electronics and information systems","ORCID":null,"PersID":35761,"InsID":2247},{"AutName":"Garrevoet","Firstname":"Jan","Initials":"J.","Affiliation":"X-Ray Microspectroscopy and Imaging Group, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":278170,"AutID":272402,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"XMI","InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Analytische Chemie; X-stralen Microspectroscopie en Beeldvorming","ORCID":null,"PersID":35195,"InsID":14069},{"AutName":"De Rijcke","Firstname":"Maarten","Initials":"M.","Affiliation":"Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":278170,"AutID":175618,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"GhEnToxLab","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Milieutoxicologie","ORCID":"0000-0002-0899-8122","PersID":25439,"InsID":6705},{"AutName":"Bauters","Firstname":"Stephen","Initials":"S.","Affiliation":"X-Ray Microspectroscopy and Imaging Group, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":278170,"AutID":259247,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"XMI","InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Analytische Chemie; X-stralen Microspectroscopie en Beeldvorming","ORCID":null,"PersID":35193,"InsID":14069},{"AutName":"Vandegehuchte","Firstname":"Michiel","Initials":"M.","Affiliation":"Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":278170,"AutID":229022,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"GhEnToxLab","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Milieutoxicologie","ORCID":"0000-0002-3915-4416","PersID":10749,"InsID":6705},{"AutName":"Van Nieuwenhove","Firstname":"Ine","Initials":"I.","Affiliation":"Polymer Chemistry and Biomaterials Group, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":278170,"AutID":259249,"OrderNr":8,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Janssen","Firstname":"Colin","Initials":"C.","Affiliation":"Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":278170,"AutID":259250,"OrderNr":9,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"GhEnToxLab","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Milieutoxicologie","ORCID":"0000-0002-7781-6679","PersID":50,"InsID":6705},{"AutName":"Burghammer","Firstname":"Manfred","Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":278170,"AutID":259251,"OrderNr":10,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":31424,"InsID":null},{"AutName":"Vincze","Firstname":"Laszlo","Initials":"L.","Affiliation":"X-Ray Microspectroscopy and Imaging Group, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":278170,"AutID":272401,"OrderNr":11,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"XMI","InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Analytische Chemie; X-stralen Microspectroscopie en Beeldvorming","ORCID":null,"PersID":35192,"InsID":14069}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":302883,"LibID":36,"BRefID":286148,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"302883","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Nature Publishing Group","City":"London"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":[{"Collection":"OMA - Open Marien Archief","ShortName":"OMA"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"},"221":{"SpName":"OMA - Open Marien Archief","SpColID":221,"ParSpColID":null,"TopParID":null,"ShortName":"OMA","URLLocation":null,"LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"OMA"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":278170,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":278170,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1038/srep09049","externalID":"10.1038/srep09049","URLTypeCode":"DOI","URLID":55789,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Bouchti, Zohra, Z.","newSesDate":{"date":"2017-06-08 08:43:17.657000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2018-03-08 08:23:07.447000","timezone_type":3,"timezone":"Europe/Brussels"}}}