{"refrec":{"BRefID":286152,"RR":"<b>Vergucht, E.; Brans, T.; Beunis, F.; Garrevoet, J.; Bauters, S.; De Rijcke, M.; Deruytter, D.; Janssen, C.; Riekel, C.; Burghammer, M.; Vincze, L.</b> (2015). Methodological challenges of optical tweezers-based X-ray fluorescence imaging of biological model organisms at synchrotron facilities. <i>Journal of Synchrotron Radiation 22(4)</i>: 1096-1105. <a href=\"https://dx.doi.org/10.1107/s1600577515009534\" target=\"_blank\">https://dx.doi.org/10.1107/s1600577515009534</a>","BEntID":278174,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Journal of Synchrotron Radiation 22(4)</i>: 1096-1105. <a href=\"https://dx.doi.org/10.1107/s1600577515009534\" target=\"_blank\">https://dx.doi.org/10.1107/s1600577515009534</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Vergucht, E.; Brans, T.; Beunis, F.; Garrevoet, J.; Bauters, S.; De Rijcke, M.; Deruytter, D.; Janssen, C.; Riekel, C.; Burghammer, M.; Vincze, L.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Vergucht, E. <i>et al.</i>","Englishabstract":"Recently, a radically new synchrotron radiation-based elemental imaging approach for the analysis of biological model organisms and single cells in their natural <i>in vivo</i> state was introduced. The methodology combines optical tweezers (OT) technology for non-contact laser-based sample manipulation with synchrotron radiation confocal X-ray fluorescence (XRF) microimaging for the first time at ESRF-ID13. The optical manipulation possibilities and limitations of biological model organisms, the OT setup developments for XRF imaging and the confocal XRF-related challenges are reported. 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