{"refrec":{"BRefID":286412,"RR":"<b>Robidart, J.; Callister, S.J.; Song, P.; Nicora, C.D.; Wheat, C.G.; Girguis, P.R.</b> (2013). Characterizing microbial community and geochemical dynamics at hydrothermal vents using osmotically driven continuous fluid samplers. <i>Environ. Sci. Technol. 47(9)</i>: 4399-4407. <a href=\"https://dx.doi.org/10.1021/es3037302\" target=\"_blank\">https://dx.doi.org/10.1021/es3037302</a>","BEntID":278436,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Environ. Sci. Technol. 47(9)</i>: 4399-4407. <a href=\"https://dx.doi.org/10.1021/es3037302\" target=\"_blank\">https://dx.doi.org/10.1021/es3037302</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Robidart, J.; Callister, S.J.; Song, P.; Nicora, C.D.; Wheat, C.G.; Girguis, P.R.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Robidart, J. <i>et al.</i>","Englishabstract":"Microbes play a key role in mediating aquatic biogeochemical cycles. However, our understanding of the relationships between microbial phylogenetic/physiological diversity and habitat physicochemical characteristics is restrained by our limited capacity to concurrently collect microbial and geochemical samples at appropriate spatial and temporal scales. Accordingly, we have developed a low-cost, continuous fluid sampling system (the Biological OsmoSampling System, or BOSS) to address this limitation. The BOSS does not use electricity, can be deployed in harsh/remote environments, and collects/preserves samples with daily resolution for >1 year. Here, we present data on the efficacy of DNA and protein preservation during a 1.5 year laboratory study as well as the results of two field deployments at deep-sea hydrothermal vents, wherein we examined changes in microbial diversity, protein expression, and geochemistry over time. Our data reveal marked changes in microbial composition co-occurring with changes in hydrothermal fluid composition as well as the temporal dynamics of an enigmatic sulfide-oxidizing symbiont in its free-living state. We also present the first data on in situ protein preservation and expression dynamics highlighting the BOSS’s potential utility in meta-proteomic studies. These data illustrate the value of using BOSS to study relationships among microbial and geochemical phenomena and environmental conditions.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Characterizing microbial community and geochemical dynamics at hydrothermal vents using osmotically driven continuous fluid samplers","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-11 01:32:18.304138","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2013,"MonPub":null,"DateUpdate":"2018-02-13","DateCreate":"2017-06-23","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000318756000057","VABBcode":null,"OpenAcc":0,"DOI":"10.1021/es3037302"},"refs":null,"anarec":{"AnaID":286412,"PubliDate":2013,"Pagination":"4399-4407","XtraPublOfAnaID":null,"ISBN":null,"Volume":"47","Issue":"9","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42837,"SerRR":"Environmental Science and Technology. 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