{"refrec":{"BRefID":286503,"RR":"<b>Pugliese, L.; Casabianca, S.; Perini, F.; Andreoni, F.; Penna, A.</b> (2017). A high resolution melting method for the molecular identification of the potentially toxic diatom <i>Pseudo-nitzschia</i> spp. in the Mediterranean Sea. <i>NPG Scientific Reports 7(1)</i>: 10 pp. <a href=\"https://dx.doi.org/10.1038/s41598-017-04245-z\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-017-04245-z</a>","BEntID":278527,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>NPG Scientific Reports 7(1)</i>: 10 pp. <a href=\"https://dx.doi.org/10.1038/s41598-017-04245-z\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-017-04245-z</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Pugliese, L.; Casabianca, S.; Perini, F.; Andreoni, F.; Penna, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Pugliese, L. <i>et al.</i>","Englishabstract":"The aim of this study was to develop and validate a high resolution melting (HRM) method for the rapid, accurate identification of the various harmful diatom <i>Pseudo-nitzschia</i> species in marine environments. <i>Pseudo-nitzschia</i> has a worldwide distribution and some species are toxic, producing the potent domoic acid toxin, which poses a threat to both human and animal health. Hence, it is important to identify toxic <i>Pseudo-nitzschia</i> species. A pair of primers targeting the LSU rDNA of the genus <i>Pseudo-nitzschia</i> was designed for the development of the assay and its specificity was validated using 22 control DNAs of the <i>P. calliantha</i>, <i>P. delicatissima/P. arenysensis</i> complex and <i>P. pungens</i>. The post-PCR HRM assay was applied to numerous unidentified <i>Pseudo-nitzschia</i> strains isolated from the northwestern Adriatic Sea (Mediterranean Sea), and it was able to detect and discriminate three distinct <i>Pseudo-nitzschia</i> taxa from unidentified samples. Moreover, the species-specific identification of <i>Pseudo-nitzschia</i> isolates by the HRM assay was consistent with phylogenetic analyses. The HRM assay was specific, robust and rapid when applied to high numbers of cultured samples in order to taxonomically identify <i>Pseudo-nitzschia</i> isolates recovered from environmental samples.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"A high resolution melting method for the molecular identification of the potentially toxic diatom <i>Pseudo-nitzschia</i> spp. in the Mediterranean Sea","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-13 01:31:32.214014","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2017,"MonPub":null,"DateUpdate":"2018-02-13","DateCreate":"2017-06-30","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000404037400026","VABBcode":null,"OpenAcc":1,"DOI":"10.1038/s41598-017-04245-z"},"refs":null,"anarec":{"AnaID":286503,"PubliDate":2017,"Pagination":"10 pp","XtraPublOfAnaID":null,"ISBN":null,"Volume":"7","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":208093,"SerRR":"Scientific Reports (Nature Publishing Group). 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ISSN 2045-2322; e-ISSN 2045-2322","StandardTitleSer":"Scientific Reports (Nature Publishing Group)","ISSN":"2045-2322","AbbrevSer":"NPG Scientific Reports","StandardTitleMon":null,"StartPage":null,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Pugliese","Firstname":"Laura","Initials":"L.","Affiliation":"Univ Urbino, Dept Biomol Sci, Viale Trieste 296, I-61121 Pesaro, Italy.","Discriminator":null,"CorporateFlag":0,"BEntID":278527,"AutID":300848,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Casabianca","Firstname":"Silvia","Initials":"S.","Affiliation":"Univ Urbino, Dept Biomol Sci, Viale Trieste 296, I-61121 Pesaro, Italy.","Discriminator":null,"CorporateFlag":0,"BEntID":278527,"AutID":300849,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Perini","Firstname":"Federico","Initials":"F.","Affiliation":"Univ Urbino, Dept Biomol Sci, Viale Trieste 296, I-61121 Pesaro, Italy.","Discriminator":null,"CorporateFlag":0,"BEntID":278527,"AutID":300850,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Andreoni","Firstname":"Francesca","Initials":"F.","Affiliation":"Univ Urbino, Dept Biomol Sci, Viale Trieste 296, I-61121 Pesaro, Italy.","Discriminator":null,"CorporateFlag":0,"BEntID":278527,"AutID":300851,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Penna","Firstname":"Antonella","Initials":"A.","Affiliation":"Univ Urbino, Dept Biomol Sci, Viale Trieste 296, I-61121 Pesaro, Italy.","Discriminator":null,"CorporateFlag":0,"BEntID":278527,"AutID":300852,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":303673,"LibID":36,"BRefID":286503,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"303673","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Nature Publishing Group","City":"London"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Pseudo-nitzschia"}],"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":278527,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":278527,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1038/s41598-017-04245-z","externalID":"10.1038/s41598-017-04245-z","URLTypeCode":"DOI","URLID":56197,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Pseudo-nitzschia","AphiaID":149151,"TaxtID":22986}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Pseudo-nitzschia","DutchTerm":null,"AphiaID":149151,"TaxtID":22986}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2017-06-30 08:23:31.697000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Lyssens, Liesbeth, L.","updSesDate":{"date":"2018-02-13 19:34:46.407000","timezone_type":3,"timezone":"Europe/Brussels"}}}