{"refrec":{"BRefID":287798,"RR":"<b>Stafsnes, M.H.; Dybwad, M.; Brunsvik, A.; Bruheim, P.</b> (2013). Large scale MALDI-TOF MS based taxa identification to identify novel pigment producers in a marine bacterial culture collection. <i>Antonie van Leeuwenhoek 103(3)</i>: 603-615. <a href=\"https://dx.doi.org/10.1007/s10482-012-9844-6\" target=\"_blank\">https://dx.doi.org/10.1007/s10482-012-9844-6</a>","BEntID":279823,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Antonie van Leeuwenhoek 103(3)</i>: 603-615. <a href=\"https://dx.doi.org/10.1007/s10482-012-9844-6\" target=\"_blank\">https://dx.doi.org/10.1007/s10482-012-9844-6</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Stafsnes, M.H.; Dybwad, M.; Brunsvik, A.; Bruheim, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Stafsnes, M.H. <i>et al.</i>","Englishabstract":"A challenge in the rational exploitation of microbial culture collections is to avoid superfluous testing of replicas. MALDI-TOF MS has been shown to be an efficient dereplication tool as it can be used to discriminate between bacterial isolates at the species level. A bacterial culture collection of more than 10,000 heterotrophic marine bacterial isolates from sea-water surface layers of the Norwegian Trondheimsfjord and neighbouring coastal areas has been established. A sub-collection of pigmented isolates was earlier screened for novel carotenoids with UVA-Blue light absorbing properties. This was a comprehensive analytical task and it was observed that a significant number of extracts with identical pigment profile were recovered. Hence, this study was undertaken to explore the use of MALDI-TOF MS as a dereplication tool to quickly characterize the bacterial collection. Furthermore, LC-DAD-MS analysis of pigment profiles was performed to check if pigment profile diversity was maintained among isolates kept after the potential MALDI-TOF MS selection step. Four hundred isolates comprising both pigmented and non-pigmented isolates were used for this study. The resulting MALDI-TOF MS dendrogram clearly identified a diversity of different taxa and these were supported by the pigment profile clustering, thus linking the pigment production as species-specific properties. Although one exception was found, it can be concluded that MALDI-TOF MS dereplication is a promising pre-screening tool for more efficient screening of microbial culture collection containing pigments with potential novel properties.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Large scale MALDI-TOF MS based taxa identification to identify novel pigment producers in a marine bacterial culture collection","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Marine bioprospecting, bacteria, Maldi-ToF MS dereplication, pigmentation, LC-MS","OtherDescriptors":null,"Notes":null,"AnaPub":2013,"MonPub":null,"DateUpdate":"2018-02-13","DateCreate":"2017-08-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000314763100017","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s10482-012-9844-6"},"refs":null,"anarec":{"AnaID":287798,"PubliDate":2013,"Pagination":"603-615","XtraPublOfAnaID":null,"ISBN":null,"Volume":"103","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42357,"SerRR":"Antonie van Leeuwenhoek. 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