{"refrec":{"BRefID":287826,"RR":"<b>Lin, J.; Kong, L.; Li, Q.</b> (2016). DNA barcoding of true limpets (Order Patellogastropoda) along coast of China: a case study. <i>Mitochondrial Dna 27(4)</i>: 2310-2314. <a href=\"https://dx.doi.org/10.3109/19401736.2015.1022758\" target=\"_blank\">https://dx.doi.org/10.3109/19401736.2015.1022758</a>","BEntID":279851,"PublicFlag":1,"CheckedFlag":0,"wosflag":null,"vabbflag":null,"RefStringPartII":". <i>Mitochondrial Dna 27(4)</i>: 2310-2314. <a href=\"https://dx.doi.org/10.3109/19401736.2015.1022758\" target=\"_blank\">https://dx.doi.org/10.3109/19401736.2015.1022758</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Lin, J.; Kong, L.; Li, Q.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Lin, J. <i>et al.</i>","Englishabstract":"In this study, we applied a partial sequence of mitochondrial <i>COI</i> gene as DNA barcode to assess the viability of DNA barcoding for distinguishing Patellogastropoda. One-hundred thirty-five <i>COI</i> gene sequences were obtained from 13 species belonging to Nacellidae (<i>Cellana</i>) and Lottiidae (<i>Lottia, Patelloida</i> and <i>Nipponacmea</i>) along the coast of China. The alignment result of these sequences indicated the existence of insertions in mitochondrial <i>COI</i> gene of Patellogastropoda. The Kimura 2-parameter (K2P) distances within species and genera were 0.00–1.01% (average 0.07%) and 18.09–37.80% (average 24.07%), respectively, an obvious barcoding gap existed. All species in our study were clearly discriminated in all trees (neighbor-joining (NJ), Bayesian, and maximum likelihood (ML) tree) with a highly supported clade node. The character-based barcode method successfully identified 100% of the Patellogastropod species included, and performed well in discriminating Patellogastropod genera. 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