{"refrec":{"BRefID":2881,"RR":"<b>Nelis, H.; Léger, P.; Sorgeloos, P.; De Leenheer, A.P.</b> (1991). Liquid chromatographic determination of efficacy of incorporation of trimethoprim and sulfamethoxazole in brine shrimp (<i>Artemia</i> spp.) used for prophylactic chemotherapy of fish. <i>Antimicrob. Agents Chemother. 35(12)</i>: 2486-2489. <a href=\"https://dx.doi.org/10.1128/AAC.35.12.2486\" target=\"_blank\">https://dx.doi.org/10.1128/AAC.35.12.2486</a>","BEntID":2881,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Antimicrob. Agents Chemother. 35(12)</i>: 2486-2489. <a href=\"https://dx.doi.org/10.1128/AAC.35.12.2486\" target=\"_blank\">https://dx.doi.org/10.1128/AAC.35.12.2486</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":1,"BrackishFlag":1,"TerrestrialFlag":0,"Authorstring":"Nelis, H.; Léger, P.; Sorgeloos, P.; De Leenheer, A.P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Nelis, H. <i>et al.</i>","Englishabstract":"The brine shrimp <i>Artemia</i> , an excellent live food source in aquaculture, has been studied as a carrier to deliver selected chemotherapeutic agents to fish for prophylactic treatment of infectious diseases. To monitor the efficiency of incorporation of trimethoprim and sulfamethoxazole in <i><i>Artemia</i> franciscana</i>, a sensitive and specific analytical method was developed. It is based on homogenization of <i>Artemia</i> nauplii in methanol, extraction of lipids with hexane, solid-phase cleanup on C<sub>18</sub> cartridges, and reversed-phase liquid chromatography with detection at 210 nm. The method is sensitive (detection limit, on the order of 3 µg/g with a sample quantity of 30 mg [dry weight]) and reproducible (coefficients of variation, 2.2 and 1.8% for trimethoprim and sulfamethoxazole at levels of 79.6 and 257 µg/g of body weight, respectively). Preliminary quantitative data indicated excellent uptake and persistence of both therapeutic agents in <i>A. franciscana</i>, with levels of 115 µg/g for trimethoprim and 277 µg/g for sulfamethoxazole.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Liquid chromatographic determination of efficacy of incorporation of trimethoprim and sulfamethoxazole in brine shrimp (<i>Artemia</i> spp.) used for prophylactic chemotherapy of fish","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-10 01:31:15.925099","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":"aquaculture techniques; fish culture; infectious diseases; medicine; <i>Artemia</i>","Notes":null,"AnaPub":1991,"MonPub":null,"DateUpdate":"2014-08-20","DateCreate":"2000-08-24","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1991GT89000006","VABBcode":null,"OpenAcc":1,"DOI":"10.1128/AAC.35.12.2486"},"refs":null,"anarec":{"AnaID":2881,"PubliDate":1991,"Pagination":"2486-2489","XtraPublOfAnaID":null,"ISBN":null,"Volume":"35","Issue":"12","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42356,"SerRR":"Antimicrobial agents and chemotherapy. 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