Document of bibliographic reference 288369

BibliographicReference record

Type

Bibliographic resource

Type of document

Journal article

BibLvlCode

AS

Title

Taxonomy assignment approach determines the efficiency of identification of OTUs in marine nematodes

Abstract

Precision and reliability of barcode-based biodiversity assessment can be affected at several steps during acquisition and analysis of data. Identification of operational taxonomic units (OTUs) is one of the crucial steps in the process and can be accomplished using several different approaches, namely, alignment-based, probabilistic, tree-based and phylogeny-based. The number of identified sequences in the reference databases affects the precision of identification. This paper compares the identification of marine nematode OTUs using alignment-based, tree-based and phylogeny-based approaches. Because the nematode reference dataset is limited in its taxonomic scope, OTUs can only be assigned to higher taxonomic categories, families. The phylogeny-based approach using the evolutionary placement algorithm provided the largest number of positively assigned OTUs and was least affected by erroneous sequences and limitations of reference data, compared to alignment-based and tree-based approaches.

WebOfScience code

https://www.webofscience.com/wos/woscc/full-record/WOS:000408764400026

Bibliographic citation

Holovachov, O.; Haenel, Q.; Bourlat, S.J.; Jondelius, U. (2017). Taxonomy assignment approach determines the efficiency of identification of OTUs in marine nematodes. Royal Society Open Science 4(8): 170315. https://dx.doi.org/10.1098/rsos.170315

Topic

Marine

Is peer reviewed

true

Access rights

open access

Is accessible for free

true