{"refrec":{"BRefID":288692,"RR":"<b>Jabbar, A.; Rashid, M.H.; Javed, M.R.; Perveen, R.; Malana, M.A.</b> (2008). Kinetics and thermodynamics of a novel endoglucanase (CMCase) from <i>Gymnoascella citrina</i> produced under solid-state condition. <i>Journal of Industrial Microbiology & Biotechnology 35(6)</i>: 515-524. <a href=\"https://dx.doi.org/10.1007/s10295-008-0310-4\" target=\"_blank\">https://dx.doi.org/10.1007/s10295-008-0310-4</a>","BEntID":280727,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Journal of Industrial Microbiology & Biotechnology 35(6)</i>: 515-524. <a href=\"https://dx.doi.org/10.1007/s10295-008-0310-4\" target=\"_blank\">https://dx.doi.org/10.1007/s10295-008-0310-4</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Jabbar, A.; Rashid, M.H.; Javed, M.R.; Perveen, R.; Malana, M.A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Jabbar, A. <i>et al.</i>","Englishabstract":"<i>Gymnoascella citrina</i> produced two isoforms of endoglucanases (CMCase-I and -ІІ) under solid-state condition. Purified CMCase-I was novel because it was apparently holoenzyme in nature. The enzyme was monomeric as its native and subunit mass were almost the same, i.e., 43 and 42 kDa, respectively. <i>E</i><sub>a</sub> for carboxymethylcellulose (CMC) hydrolysis was 36.2 kJ mol<sup>−1</sup>. The enzyme was stable over a pH range of 3.5–6.5, while temperature optimum was 55 °C. <i>V</i><sub>max</sub>, <i>K</i><sub>m </sub>and <i>k</i><sub>cat </sub>for CMC hydrolysis were 39 U mg<sup>−1</sup> protein, 6.25 mg CMC mL<sup>−1</sup> and 27.5 s<sup>−1</sup>, respectively. The p<i>K</i>a<sub>1</sub> and p<i>K</i>a<sub>2 </sub>of ionizable groups of active site were 2.8 and 7.4, respectively. Thermodynamic parameters for CMC hydrolysis were as follows: ΔH<sup>*</sup> = 33.5 kJ mol<sup>−1</sup>, ΔG<sup>*</sup> = 70.42 kJ mol<sup>−1</sup> and ΔS<sup>*</sup> = −114.37 J mol<sup>−1</sup> K<sup>−1</sup>. The removal of metals resulted into complete loss of enzymatic activity and was completely recovered in the presence of 1 mM Mn<sup>2+</sup>, whereas inhibition initiated at 5 mM. The other metals like Ca<sup>2+</sup>, Zn<sup>2+</sup> and K<sup>1+ </sup>showed no inhibition up to 7 mM, Co<sup>2+</sup> completely inhibited the activity, while Mg<sup>2+</sup> could not recover the initial activity up to 7 mM. So we are reporting for the first time, kinetics and thermodynamics of CMCase-Ι from <i>G. citrina</i>.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Kinetics and thermodynamics of a novel endoglucanase (CMCase) from <i>Gymnoascella citrina</i> produced under solid-state condition","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Endoglucanase, Activation energy, Enthalpy, Entropy, Gibbs free energy","OtherDescriptors":null,"Notes":null,"AnaPub":2008,"MonPub":null,"DateUpdate":"2017-10-20","DateCreate":"2017-08-24","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000255863000008","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s10295-008-0310-4"},"refs":null,"anarec":{"AnaID":288692,"PubliDate":2008,"Pagination":"515-524","XtraPublOfAnaID":null,"ISBN":null,"Volume":"35","Issue":"6","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":272243,"SerRR":"Journal of Industrial Microbiology & Biotechnology. 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ISSN 1367-5435; e-ISSN 1476-5535","StandardTitleSer":"Journal of Industrial Microbiology & Biotechnology","ISSN":"1367-5435","AbbrevSer":null,"StandardTitleMon":null,"StartPage":515,"Pages":10,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Jabbar","Firstname":"Abdul","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":280727,"AutID":268578,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Rashid","Firstname":"Muhammad Hamid","Initials":"M.H.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":280727,"AutID":268579,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Javed","Firstname":"Muhammad Rizwan","Initials":"M.R.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":280727,"AutID":268580,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Perveen","Firstname":"Raheela","Initials":"R.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":280727,"AutID":268581,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Malana","Firstname":"Muhammad Aslam","Initials":"M.A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":280727,"AutID":268582,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":308101,"LibID":36,"BRefID":288692,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"308101","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"SPRINGER HEIDELBERG","City":"Heidelberg"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"955":{"SpName":"Catalogue of Life acknowledged","SpColID":955,"ParSpColID":null,"TopParID":null,"ShortName":"Catalogue of Life ackn","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"Catalogue of Life ackn"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":280727,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":280727,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1007/s10295-008-0310-4","externalID":"10.1007/s10295-008-0310-4","URLTypeCode":"DOI","URLID":57592,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"VLIZ2000\\sielc","newSesDate":{"date":"2017-08-24 08:11:44.943000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2017-10-20 08:30:31.767000","timezone_type":3,"timezone":"Europe/Brussels"}}}