{"refrec":{"BRefID":289029,"RR":"<b>Le Garrec, R.; L'herondelle, K.; Le Gall-Ianotto, C.; Lebonvallet, N.; Leschiera, R.; Buhe, V.; Talagas, M.; Vetter, I.; Lewis, R.J.; Misery, L.</b> (2016). Release of neuropeptides from a neuro-cutaneous co-culture model: a novel <i>in vitro</i> model for studying sensory effects of ciguatoxins. <i>Toxicon 116</i>: 4-10. <a href=\"https://dx.doi.org/10.1016/j.toxicon.2015.11.004\" target=\"_blank\">https://dx.doi.org/10.1016/j.toxicon.2015.11.004</a>","BEntID":281065,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Toxicon 116</i>: 4-10. <a href=\"https://dx.doi.org/10.1016/j.toxicon.2015.11.004\" target=\"_blank\">https://dx.doi.org/10.1016/j.toxicon.2015.11.004</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Le Garrec, R.; L'herondelle, K.; Le Gall-Ianotto, C.; Lebonvallet, N.; Leschiera, R.; Buhe, V.; Talagas, M.; Vetter, I.; Lewis, R.J.; Misery, L.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Le Garrec, R. <i>et al.</i>","Englishabstract":"Ciguatoxins are the major toxins responsible for ciguatera fish poisoning, a disease dominated by muco-cutaneous sensory disorders including paresthesiae, cold dysesthesia and pruritus. While the ciguatoxins are well known to target voltage-gated sodium channels (VGSCs), the ensuing molecular mechanisms underlying these sensory disorders remain poorly understood. In this study, we propose a primary sensory neuron-keratinocyte co-culture as an appropriate model to study the neuro-cutaneous effects of ciguatoxins. Using this model, we show for the first time that nanomolar concentrations of Pacific ciguatoxin-2 (P-CTX-2) induced a VGSC-dependent release of substance P (SP) and calcitonin gene-related peptide (CGRP). As these neuropeptides are known mediators of pain and itch sensations, the ciguatoxin-induced sensory disturbances in ciguatera fish poisoning may involve the release of these neuropeptides. We further determined time- and P-CTX-2 concentration-dependence of the release of SP and CGRP from the co-culture model. Moreover, we highlighted the influence of extracellular calcium on the release of neuropeptides elicited by P-CTX-2. These findings underline the usefulness of this novel <i>in vitro</i> model for studying the cellular and molecular mechanisms of the neuro-cutaneous effects of ciguatoxins, which may assist with identifying potential therapeutics for ciguatera fish poisoning.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Release of neuropeptides from a neuro-cutaneous co-culture model: a novel <i>in vitro</i> model for studying sensory effects of ciguatoxins","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Ciguatoxins Sensory disorders Neuropeptide release In vitro model","OtherDescriptors":null,"Notes":null,"AnaPub":2016,"MonPub":null,"DateUpdate":"2018-02-13","DateCreate":"2017-09-07","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000376830400002","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.toxicon.2015.11.004"},"refs":null,"anarec":{"AnaID":289029,"PubliDate":2016,"Pagination":"4-10","XtraPublOfAnaID":null,"ISBN":null,"Volume":"116","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43961,"SerRR":"Toxicon. 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