{"refrec":{"BRefID":289220,"RR":"<b>Godfroy, O.; Peters, A.F.; Coelho, S.M.; Cock, J.M.</b> (2015). Genome-wide comparison of ultraviolet and ethyl methanesulphonate mutagenesis methods for the brown alga <i>Ectocarpus</i>. <i>Marine Genomics 24(1)</i>: 109-113. <a href=\"https://dx.doi.org/10.1016/j.margen.2015.03.007\" target=\"_blank\">https://dx.doi.org/10.1016/j.margen.2015.03.007</a>","BEntID":281257,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Marine Genomics 24(1)</i>: 109-113. <a href=\"https://dx.doi.org/10.1016/j.margen.2015.03.007\" target=\"_blank\">https://dx.doi.org/10.1016/j.margen.2015.03.007</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Godfroy, O.; Peters, A.F.; Coelho, S.M.; Cock, J.M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Godfroy, O. <i>et al.</i>","Englishabstract":"<i>Ectocarpus</i> has emerged as a model organism for the brown algae and a broad range of genetic and genomic resources are being generated for this species. The aim of the work presented here was to evaluate two mutagenesis protocols based on ultraviolet irradiation and ethyl methanesulphonate treatment using genome resequencing to measure the number, type and distribution of mutations generated by the two methods. Ultraviolet irradiation generated a greater number of genetic lesions than ethyl methanesulphonate treatment, with more than 400 mutations being detected in the genome of the mutagenised individual. This study therefore confirms that the ultraviolet mutagenesis protocol is suitable for approaches that require a high density of mutations, such as saturation mutagenesis or Targeting Induced Local Lesions in Genomes (TILLING).","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Genome-wide comparison of ultraviolet and ethyl methanesulphonate mutagenesis methods for the brown alga <i>Ectocarpus</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Brown algae; Ectocarpus; Ethyl methanesulphonate; Mutagenesis;Ultraviolet","OtherDescriptors":null,"Notes":null,"AnaPub":2015,"MonPub":null,"DateUpdate":"2017-09-20","DateCreate":"2017-09-18","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000367774900013","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.margen.2015.03.007"},"refs":null,"anarec":{"AnaID":289220,"PubliDate":2015,"Pagination":"109-113","XtraPublOfAnaID":null,"ISBN":null,"Volume":"24","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":126432,"SerRR":"Marine Genomics. Elsevier: Amsterdam.  ISSN 1874-7787; e-ISSN 1876-7478","StandardTitleSer":"Marine Genomics","ISSN":"1874-7787","AbbrevSer":"Marine Genomics","StandardTitleMon":null,"StartPage":109,"Pages":5,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Godfroy","Firstname":"Olivier","Initials":"O.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":281257,"AutID":270557,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Peters","Firstname":"Akira","Initials":"A.F.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":281257,"AutID":126337,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Coelho","Firstname":"Susana","Initials":"S.M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":281257,"AutID":71160,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Cock","Firstname":"J. Mark","Initials":"J.M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":281257,"AutID":71158,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":306946,"LibID":36,"BRefID":289220,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"306946","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier","City":"Amsterdam"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Exploitable Scientific Result","ThestID":169539,"Acronym":"KTPKO","ThesTermPath":"Exploitable Scientific Result"},{"ThesaurusTerm":"Marine Sciences","ThestID":169563,"Acronym":"KTPS","ThesTermPath":"Marine Sciences"},{"ThesaurusTerm":"Blue Biotech","ThestID":169589,"Acronym":"KTPS","ThesTermPath":"Maritime Industries > Blue Biotech"},{"ThesaurusTerm":"Scientific Community","ThestID":169557,"Acronym":"KTPUT","ThesTermPath":"Scientific Community"},{"ThesaurusTerm":"Scientific Publication","ThestID":169548,"Acronym":"KTPKO","ThesTermPath":"Scientific Publication"}],"thestermsASFA":[{"ThesaurusTerm":"Blue Biotech"},{"ThesaurusTerm":"Exploitable Scientific Result"},{"ThesaurusTerm":"Marine Sciences"},{"ThesaurusTerm":"Scientific Community"},{"ThesaurusTerm":"Scientific Publication"}],"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":[{"ProID":4198,"Acronym":"ASSEMBLE","ProStandardTitle":"Association of European marine biological laboratories","BYear":2009,"BMonth":3,"EYear":2013,"EMonth":2,"FP7Code":"A-2008","RelID":null,"Relation":null,"DutchTerm":null}],"Physdatasets":null,"spcols":{"990":{"SpName":"Assemble","SpColID":990,"ParSpColID":null,"TopParID":null,"ShortName":"Assemble","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"Assemble"},"951":{"SpName":"AssembleMarine","SpColID":951,"ParSpColID":990,"TopParID":990,"ShortName":"AssembleMarine","URLLocation":null,"LibID":null,"OpenRepoFlag":0,"SpTypID":null,"TopParIDNotWebsite":990,"SpColPath":"Assemble/AssembleMarine"},"950":{"SpName":"EMBRC","SpColID":950,"ParSpColID":null,"TopParID":null,"ShortName":"EMBRC","URLLocation":null,"LibID":null,"OpenRepoFlag":0,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"EMBRC"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":281257,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":281257,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/j.margen.2015.03.007","externalID":"10.1016/j.margen.2015.03.007","URLTypeCode":"DOI","URLID":58096,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"Blue Biotech","ThestID":169589,"Acronym":"KTPS","ThesTypID":10,"ThesType":"Assemble Sectors"},{"ThesaurusTerm":"Exploitable Scientific Result","ThestID":169539,"Acronym":"KTPKO","ThesTypID":8,"ThesType":"Assemble KO Types"},{"ThesaurusTerm":"Marine Sciences","ThestID":169563,"Acronym":"KTPS","ThesTypID":10,"ThesType":"Assemble Sectors"},{"ThesaurusTerm":"Scientific Community","ThestID":169557,"Acronym":"KTPUT","ThesTypID":9,"ThesType":"Assemble User Types"},{"ThesaurusTerm":"Scientific Publication","ThestID":169548,"Acronym":"KTPKO","ThesTypID":8,"ThesType":"Assemble KO Types"}],"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Blue Biotech","DutchTerm":null,"ThestID":169589,"Acronym":"KTPS","ThesTypID":10,"ThesType":"Assemble Sectors"},{"ThesaurusTerm":"Exploitable Scientific Result","DutchTerm":null,"ThestID":169539,"Acronym":"KTPKO","ThesTypID":8,"ThesType":"Assemble KO Types"},{"ThesaurusTerm":"Marine Sciences","DutchTerm":null,"ThestID":169563,"Acronym":"KTPS","ThesTypID":10,"ThesType":"Assemble Sectors"},{"ThesaurusTerm":"Scientific Community","DutchTerm":null,"ThestID":169557,"Acronym":"KTPUT","ThesTypID":9,"ThesType":"Assemble User Types"},{"ThesaurusTerm":"Scientific Publication","DutchTerm":null,"ThestID":169548,"Acronym":"KTPKO","ThesTypID":8,"ThesType":"Assemble KO Types"}],"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2017-09-18 22:10:53.193000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2017-09-20 08:31:24.910000","timezone_type":3,"timezone":"Europe/Brussels"}}}