{"refrec":{"BRefID":289239,"RR":"<b>Bendif, E.M.; Probert, I.; Young, J.R.; von Dassow, P.</b> (2015). Morphological and phylogenetic characterization of new <i>Gephyrocapsa</i> isolates suggests introgressive hybridization in the <i>Emiliania/Gephyrocapsa</i> complex (Haptophyta). <i>Protist 166(3)</i>: 323-336. <a href=\"https://dx.doi.org/10.1016/j.protis.2015.05.003\" target=\"_blank\">https://dx.doi.org/10.1016/j.protis.2015.05.003</a>","BEntID":281276,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Protist 166(3)</i>: 323-336. <a href=\"https://dx.doi.org/10.1016/j.protis.2015.05.003\" target=\"_blank\">https://dx.doi.org/10.1016/j.protis.2015.05.003</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Bendif, E.M.; Probert, I.; Young, J.R.; von Dassow, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Bendif, E.M. <i>et al.</i>","Englishabstract":"The coccolithophore genus <i>Gephyrocapsa</i> contains a cosmopolitan assemblage of pelagic species, including the bloom-forming <i>Gephyrocapsa oceanica</i>, and is closely related to the emblematic coccolithophore <i>Emiliania huxleyi</i> within the Noëlaerhabdaceae. These two species have been extensively studied and are well represented in culture collections, whereas cultures of other species of this family are lacking. We report on three new strains of <i>Gephyrocapsa</i> isolated into culture from samples from the Chilean coastal upwelling zone using a novel flow cytometric single-cell sorting technique. The strains were characterized by morphological analysis using scanning electron microscopy and phylogenetic analysis of 6 genes (nuclear 18S and 28S rDNA, plastidial 16S and <i>tufA</i>, and mitochondrial cox1 and cox3 genes). Morphometric features of the coccoliths indicate that these isolates are distinct from <i>G. oceanica</i> and best correspond to <i>G. muellerae</i>. Surprisingly, both plastidial and mitochondrial gene phylogenies placed these strains within the <i>E. huxleyi</i> clade and well separated from <i>G. oceanica</i> isolates, making <i>Emiliania</i> appear polyphyletic. The only nuclear sequence difference, 1 bp in the 28S rDNA region, also grouped <i>E. huxleyi</i> with the new <i>Gephyrocapsa</i> isolates and apart from <i>G. oceanica</i>. Specifically, the <i>G. muellerae</i> morphotype strains clustered with the mitochondrial β clade of <i>E. huxleyi</i>, which, like <i>G. muellerae</i>, has been associated with cold (temperate and sub-polar) waters. Among putative evolutionary scenarios that could explain these results we discuss the possibility that <i>E. huxleyi</i> is not a valid taxonomic unit, or, alternatively the possibility of past hybridization and introgression between each <i>E. huxleyi</i> clade and older <i>Gephyrocapsa</i> clades. In either case, the results support the transfer of <i>Emiliania</i> to <i>Gephyrocapsa</i>. These results have important implications for relating morphological species concepts to ecological and evolutionary units of diversity.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Morphological and phylogenetic characterization of new <i>Gephyrocapsa</i> isolates suggests introgressive hybridization in the <i>Emiliania/Gephyrocapsa</i> complex (Haptophyta)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Coccolithophores; Emiliania huxleyi; Gephyrocapsa muellerae;Gephyrocapsa oceanica; hybridization; species concept; phylogeny","OtherDescriptors":null,"Notes":null,"AnaPub":2015,"MonPub":null,"DateUpdate":"2017-09-20","DateCreate":"2017-09-18","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000358085700003","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.protis.2015.05.003"},"refs":null,"anarec":{"AnaID":289239,"PubliDate":2015,"Pagination":"323-336","XtraPublOfAnaID":null,"ISBN":null,"Volume":"166","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45701,"SerRR":"Protist. 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