{"refrec":{"BRefID":289278,"RR":"<b>Kalampoki, L.G.; Flytzanis, C.N.</b> (2014). <i>Cis</i>-regulatory control of the nuclear receptor <i>Coup-TF</i> gene in the sea urchin <i>Paracentrotus lividus</i> embryo. <i>PLoS One 9(11)</i>: e109274. <a href=\"https://dx.doi.org/10.1371/journal.pone.0109274\" target=\"_blank\">https://dx.doi.org/10.1371/journal.pone.0109274</a>","BEntID":281315,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>PLoS One 9(11)</i>: e109274. <a href=\"https://dx.doi.org/10.1371/journal.pone.0109274\" target=\"_blank\">https://dx.doi.org/10.1371/journal.pone.0109274</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Kalampoki, L.G.; Flytzanis, C.N.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Kalampoki, L.G.; Flytzanis, C.N.","Englishabstract":"Coup-TF, an orphan member of the nuclear receptor super family, has a fundamental role in the development of metazoan embryos. The study of the gene's regulatory circuit in the sea urchin embryo will facilitate the placement of this transcription factor in the well-studied embryonic Gene Regulatory Network (GRN). The <i>Paracentrotus lividus Coup-TF</i> gene (<i>PlCoup-TF</i>) is expressed throughout embryonic development preferentially in the oral ectoderm of the gastrula and the ciliary band of the pluteus stage. Two overlapping λ genomic clones, containing three exons and upstream sequences of <i>PlCoup-TF</i>, were isolated from a genomic library. The transcription initiation site was determined and 5′ deletions and individual segments of a 1930 bp upstream region were placed ahead of a GFP reporter cassette and injected into fertilized <i>P.lividus</i> eggs. Module <i>a</i> (−532 to −232), was necessary and sufficient to confer ciliary band expression to the reporter. Comparison of <i>P.lividus</i> and <i>Strongylocentrotus purpuratus</i> upstream <i>Coup-TF</i> sequences, revealed considerable conservation, but none within module <i>a</i>. 5′ and internal deletions into module <i>a</i>, defined a smaller region that confers ciliary band specific expression. Putative regulatory <i>cis</i>-acting elements (RE1, RE2 and RE3) within module <i>a</i>, were specifically bound by proteins in sea urchin embryonic nuclear extracts. Site-specific mutagenesis of these elements resulted in loss of reporter activity (RE1) or ectopic expression (RE2, RE3). It is proposed that sea urchin transcription factors, which bind these three regulatory sites, are necessary for spatial and quantitative regulation of the <i>PlCoup-TF</i> gene at pluteus stage sea urchin embryos. These findings lead to the future identification of these factors and to the hierarchical positioning of PlCoup-TF within the embryonic GRN.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"<i>Cis</i>-regulatory control of the nuclear receptor <i>Coup-TF</i> gene in the sea urchin <i>Paracentrotus lividus</i> embryo","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:01.897972","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2014,"MonPub":null,"DateUpdate":"2017-09-19","DateCreate":"2017-09-18","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000345250400002","VABBcode":null,"OpenAcc":1,"DOI":"10.1371/journal.pone.0109274"},"refs":null,"anarec":{"AnaID":289278,"PubliDate":2014,"Pagination":"e109274","XtraPublOfAnaID":null,"ISBN":null,"Volume":"9","Issue":"11","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":123954,"SerRR":"PLoS One. 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