{"refrec":{"BRefID":289288,"RR":"<b>Memmola, F.; Mukherjee, B.; Moroney, J.V.; Giordano, M.</b> (2014). Carbon allocation and element composition in four <i>Chlamydomonas</i> mutants defective in genes related to the CO<sub>2</sub> concentrating mechanism. <i>Photosynth. Res. 121(2-3)</i>: 201-211. <a href=\"https://dx.doi.org/10.1007/s11120-014-0005-9\" target=\"_blank\">https://dx.doi.org/10.1007/s11120-014-0005-9</a>","BEntID":281325,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Photosynth. Res. 121(2-3)</i>: 201-211. <a href=\"https://dx.doi.org/10.1007/s11120-014-0005-9\" target=\"_blank\">https://dx.doi.org/10.1007/s11120-014-0005-9</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Memmola, F.; Mukherjee, B.; Moroney, J.V.; Giordano, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Memmola, F. <i>et al.</i>","Englishabstract":"Four mutants of <i>Chlamydomonas reinhardtii</i> with defects in different components of the CO<sub>2</sub> concentrating mechanism (CCM) or in Rubisco activase were grown autotrophically at high pCO<sub>2</sub> and then transferred to low pCO<sub>2</sub>, in order to study the role of different components of the CCM on carbon allocation and elemental composition. To study carbon allocation, we measured the relative size of the main organic pools by Fourier Transform Infrared spectroscopy. Total reflection X-ray fluorescence was used to analyze the elemental composition of algal cells. Our data show that although the organic pools increased their size at high CO<sub>2</sub> in all strains, their stoichiometry was highly homeostatic, i.e., the ratios between carbohydrates and proteins, lipid and proteins, and carbohydrates and lipids, did not change significantly. The only exception was the wild-type 137c, in which proteins decreased relative to carbohydrates and lipids, when the cells were transferred to low CO<sub>2</sub>. It is noticeable that the two wild types used in this study responded differently to the transition from high to low CO<sub>2</sub>. Malfunctions of the CCM influenced the concentration of several elements, somewhat altering cell elemental stoichiometry: especially the C/P and N/P ratios changed appreciably in almost all strains as a function of the growth CO<sub>2</sub> concentration, except in 137c and the Rubisco activase mutant rca1. In strain cia3, defective in the lumenal carbonic anhydrase (CA), the cell quotas of P, S, Ca, Mn, Fe, and Zn were about 5-fold higher at low CO<sub>2</sub> than at high CO<sub>2</sub>. A Principle Components Analysis showed that, mostly because of its elemental composition, cia3 behaved in a substantially different way from all other strains, at low CO<sub>2</sub>. The lumenal CA thus plays a crucial role, not only for the correct functioning of the CCM, but also for element utilization. Not surprisingly, growth at high CO<sub>2</sub> attenuated differences among strains.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Carbon allocation and element composition in four <i>Chlamydomonas</i> mutants defective in genes related to the CO<sub>2</sub> concentrating mechanism","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-22 01:31:58.916949","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Carbon allocation; CCM; Chlamydomonas; Cell organic composition; CO2concentrating mechanisms; Elemental stoichiometry","OtherDescriptors":null,"Notes":null,"AnaPub":2014,"MonPub":null,"DateUpdate":"2017-09-20","DateCreate":"2017-09-18","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000338763500010","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s11120-014-0005-9"},"refs":null,"anarec":{"AnaID":289288,"PubliDate":2014,"Pagination":"201-211","XtraPublOfAnaID":null,"ISBN":null,"Volume":"121","Issue":"2-3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":240562,"SerRR":"Photosynthesis Research. 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